High-performance liquid chromatographic analysis of as-synthesised N,N′-dimethylformamide-stabilised gold nanoclusters product

Shunping Xie, Man Chin Paau, Yan Zhang, Shaomin Shuang*, Wan Chan, Martin M F CHOI

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

Reverse-phase high-performance liquid chromatographic (RP-HPLC) separation and analysis of polydisperse water-soluble gold nanoclusters (AuNCs) stabilised with N,N′-dimethylformamide (DMF) were investigated. Under optimal elution gradient conditions, the separation of DMF-AuNCs was monitored by absorption and fluorescence spectroscopy. The UV-vis spectral characteristics of the separated DMF-AuNCs have been captured and they do not possess distinct surface plasmon resonance bands, indicating that all DMF-AuNCs are small AuNCs. The photoluminescence emission spectra of the separated DMF-AuNCs are in the blue-light region. Moreover, cationic DMF-AuNCs are for the first time identified by ion chromatography. Our proposed RP-HPLC methodology has been successfully applied to separate AuNCs of various Au atoms as well as DMF-stabilised ligands. Finally, the composition of the separated DMF-AuNCs was confirmed by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry and electrospray ionisation mass spectrometry, proving that the as-synthesised DMF-AuNCs product consists of Au10+, Au10, Au11, Au12, Au13, and Au 14 NCs stabilised with various numbers of DMF ligands.

Original languageEnglish
Pages (from-to)5325-5332
Number of pages8
JournalNanoscale
Volume4
Issue number17
DOIs
Publication statusPublished - 7 Sep 2012

Scopus Subject Areas

  • Materials Science(all)

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