High expression of SARS-CoV-2 entry factors in human conjunctival goblet cells

Ruiqi Ma; Lu Gan; Sanjie Jiang; Peiwen Ding; Dongsheng Chen; Jihong Wu; Jiang Qian

doi:10.1016/j.exer.2021.108501

High expression of SARS-CoV-2 entry factors in human conjunctival goblet cells

Ruiqi Ma
, Lu Gan
, Sanjie Jiang
, Peiwen Ding
, Dongsheng Chen^*
, Jihong Wu^*
, Jiang Qian^*

^*Corresponding author for this work

Department of Biology

Research output: Contribution to journal › Journal article › peer-review

12 Citations (Scopus)

Abstract

The angiotensin-converting enzyme 2 (ACE2) receptor has been proved for SARS-CoV-2 cell entry after auxiliary cellular protease priming by transmembrane protease serine 2 (TMPRSS2), but the co-effect of this molecular mechanism was unknown. Here, single-cell sequencing was performed with human conjunctiva and the results have shown that ACE2 and TMPRSS2 were highly co-expressed in the goblet cells with genes involved in immunity process. This identification of conjunctival cell types which are permissive to virus entry would help to understand the process by which SARS-CoV-2 infection was established. These finding might be suggestive for COVID-19 control and protection.

Original language	English
Article number	108501
Number of pages	4
Journal	Experimental Eye Research
Volume	205
DOIs	https://doi.org/10.1016/j.exer.2021.108501
Publication status	Published - Apr 2021

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This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

User-Defined Keywords

SARS-CoV-2
Conjunctival goblet cell
ACE2
TMPRSS2

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10.1016/j.exer.2021.108501

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@article{9bf399ad08934b8eb74c144a7023772e,

title = "High expression of SARS-CoV-2 entry factors in human conjunctival goblet cells",

abstract = "The angiotensin-converting enzyme 2 (ACE2) receptor has been proved for SARS-CoV-2 cell entry after auxiliary cellular protease priming by transmembrane protease serine 2 (TMPRSS2), but the co-effect of this molecular mechanism was unknown. Here, single-cell sequencing was performed with human conjunctiva and the results have shown that ACE2 and TMPRSS2 were highly co-expressed in the goblet cells with genes involved in immunity process. This identification of conjunctival cell types which are permissive to virus entry would help to understand the process by which SARS-CoV-2 infection was established. These finding might be suggestive for COVID-19 control and protection.",

keywords = "SARS-CoV-2, Conjunctival goblet cell, ACE2, TMPRSS2",

author = "Ruiqi Ma and Lu Gan and Sanjie Jiang and Peiwen Ding and Dongsheng Chen and Jihong Wu and Jiang Qian",

note = "Publisher Copyright: {\textcopyright} 2021 The Authors. Published by Elsevier Ltd. Funding Information: JQ was supported by the National Natural Science Foundation of China (grant number 81970835, 81800867) and the Natural Science Foundation of Shanghai (grant number 20ZR1409500). JHW was supported by Outstanding Academic Leaders in Shanghai (20XD1401100), Program for Outstanding Medical Academic Leader (2019LJ01), Aging and Women{\textquoteright}s and Children{\textquoteright}s Health Special Project of Shanghai Municipal Health Commission (2020YJZX0102), Xuhui District Health and Family Planning Commission Key Disease Joint Project (XHLHGG201807).",

year = "2021",

month = apr,

doi = "10.1016/j.exer.2021.108501",

language = "English",

volume = "205",

journal = "Experimental Eye Research",

issn = "0014-4835",

publisher = "Academic Press",

}

TY - JOUR

T1 - High expression of SARS-CoV-2 entry factors in human conjunctival goblet cells

AU - Ma, Ruiqi

AU - Gan, Lu

AU - Jiang, Sanjie

AU - Ding, Peiwen

AU - Chen, Dongsheng

AU - Wu, Jihong

AU - Qian, Jiang

N1 - Publisher Copyright: © 2021 The Authors. Published by Elsevier Ltd. Funding Information: JQ was supported by the National Natural Science Foundation of China (grant number 81970835, 81800867) and the Natural Science Foundation of Shanghai (grant number 20ZR1409500). JHW was supported by Outstanding Academic Leaders in Shanghai (20XD1401100), Program for Outstanding Medical Academic Leader (2019LJ01), Aging and Women’s and Children’s Health Special Project of Shanghai Municipal Health Commission (2020YJZX0102), Xuhui District Health and Family Planning Commission Key Disease Joint Project (XHLHGG201807).

PY - 2021/4

Y1 - 2021/4

N2 - The angiotensin-converting enzyme 2 (ACE2) receptor has been proved for SARS-CoV-2 cell entry after auxiliary cellular protease priming by transmembrane protease serine 2 (TMPRSS2), but the co-effect of this molecular mechanism was unknown. Here, single-cell sequencing was performed with human conjunctiva and the results have shown that ACE2 and TMPRSS2 were highly co-expressed in the goblet cells with genes involved in immunity process. This identification of conjunctival cell types which are permissive to virus entry would help to understand the process by which SARS-CoV-2 infection was established. These finding might be suggestive for COVID-19 control and protection.

AB - The angiotensin-converting enzyme 2 (ACE2) receptor has been proved for SARS-CoV-2 cell entry after auxiliary cellular protease priming by transmembrane protease serine 2 (TMPRSS2), but the co-effect of this molecular mechanism was unknown. Here, single-cell sequencing was performed with human conjunctiva and the results have shown that ACE2 and TMPRSS2 were highly co-expressed in the goblet cells with genes involved in immunity process. This identification of conjunctival cell types which are permissive to virus entry would help to understand the process by which SARS-CoV-2 infection was established. These finding might be suggestive for COVID-19 control and protection.

KW - SARS-CoV-2

KW - Conjunctival goblet cell

KW - ACE2

KW - TMPRSS2

UR - https://www.scopus.com/pages/publications/85101416922

U2 - 10.1016/j.exer.2021.108501

DO - 10.1016/j.exer.2021.108501

M3 - Journal article

C2 - 33600811

AN - SCOPUS:85101416922

SN - 0014-4835

VL - 205

JO - Experimental Eye Research

JF - Experimental Eye Research

M1 - 108501

ER -

High expression of SARS-CoV-2 entry factors in human conjunctival goblet cells

Abstract

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