Herbal isoprenols induce apoptosis in human colon cancer cells through transcriptional activation of PPARγ

Kathy K W AU YEUNG; Po Ling Liu; Cecilia Chan; Wing Yee Wu; Susanna Sau Tuen Lee; Joshua KO

doi:10.1080/07357900801898656

Herbal isoprenols induce apoptosis in human colon cancer cells through transcriptional activation of PPARγ

Kathy K W AU YEUNG, Po Ling Liu, Cecilia Chan, Wing Yee Wu, Susanna Sau Tuen Lee, Joshua KO

Research output: Contribution to journal › Journal article › peer-review

28 Citations (Scopus)

Abstract

Farnesol (FOH) and geranylgeraniol (GGOH) possess anti-tumor potential, while peroxisome proliferator-activated receptor γ (PPARγ) has exhibited modulating effects in colorectal cancers. We investigated the anti-carcinogenic effects of these isoprenols in HT-29 and HCT116 colon cancer cells and PPARγ involvement. Results indicate that the FOH- and GGOH-induced apoptosis involve caspase 3 activation, PARP cleavage, nuclear chromatin condensation, down-regulation of Bcl-xL and survivin expression, with increased PPARγ promoter activity. Pretreatment of the PPARγ antagonist GW9662 reduces FOH-induced growth inhibition and the associated PARP cleavage. We conclude that PPARγ activation is essential to elicit the anti-carcinogenic action of herbal isoprenols in colonic cancer cells.

Original language	English
Pages (from-to)	708-717
Number of pages	10
Journal	Cancer Investigation
Volume	26
Issue number	7
DOIs	https://doi.org/10.1080/07357900801898656
Publication status	Published - Aug 2008

Scopus Subject Areas

Oncology
Cancer Research

User-Defined Keywords

FOH
GGOH
GW9662
HCT116 cells
HT-29 cells
PPARγ

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1080/07357900801898656

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@article{9914e850770745b2875555c65589a45b,

title = "Herbal isoprenols induce apoptosis in human colon cancer cells through transcriptional activation of PPARγ",

abstract = "Farnesol (FOH) and geranylgeraniol (GGOH) possess anti-tumor potential, while peroxisome proliferator-activated receptor γ (PPARγ) has exhibited modulating effects in colorectal cancers. We investigated the anti-carcinogenic effects of these isoprenols in HT-29 and HCT116 colon cancer cells and PPARγ involvement. Results indicate that the FOH- and GGOH-induced apoptosis involve caspase 3 activation, PARP cleavage, nuclear chromatin condensation, down-regulation of Bcl-xL and survivin expression, with increased PPARγ promoter activity. Pretreatment of the PPARγ antagonist GW9662 reduces FOH-induced growth inhibition and the associated PARP cleavage. We conclude that PPARγ activation is essential to elicit the anti-carcinogenic action of herbal isoprenols in colonic cancer cells.",

keywords = "FOH, GGOH, GW9662, HCT116 cells, HT-29 cells, PPARγ",

author = "{AU YEUNG}, {Kathy K W} and Liu, {Po Ling} and Cecilia Chan and Wu, {Wing Yee} and Lee, {Susanna Sau Tuen} and Joshua KO",

note = "Funding Information: Keywords: FOH, GGOH, HT-29 cells, HCT116 cells, PPARγ , GW9662. This study was supported by the Hong Kong Baptist University Faculty Research Grant (FRG/05-06/II-21). Correspondence to: Joshua Ka-shun Ko, Ph.D. Pharmacology and Toxicology Laboratory School of Chinese Medicine Hong Kong Baptist University 7 Baptist University Road Kowloon Tong, Hong Kong China email: [email protected]",

year = "2008",

month = aug,

doi = "10.1080/07357900801898656",

language = "English",

volume = "26",

pages = "708--717",

journal = "Cancer Investigation",

issn = "0735-7907",

publisher = "Informa Healthcare",

number = "7",

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TY - JOUR

T1 - Herbal isoprenols induce apoptosis in human colon cancer cells through transcriptional activation of PPARγ

AU - AU YEUNG, Kathy K W

AU - Liu, Po Ling

AU - Chan, Cecilia

AU - Wu, Wing Yee

AU - Lee, Susanna Sau Tuen

AU - KO, Joshua

N1 - Funding Information: Keywords: FOH, GGOH, HT-29 cells, HCT116 cells, PPARγ , GW9662. This study was supported by the Hong Kong Baptist University Faculty Research Grant (FRG/05-06/II-21). Correspondence to: Joshua Ka-shun Ko, Ph.D. Pharmacology and Toxicology Laboratory School of Chinese Medicine Hong Kong Baptist University 7 Baptist University Road Kowloon Tong, Hong Kong China email: [email protected]

PY - 2008/8

Y1 - 2008/8

N2 - Farnesol (FOH) and geranylgeraniol (GGOH) possess anti-tumor potential, while peroxisome proliferator-activated receptor γ (PPARγ) has exhibited modulating effects in colorectal cancers. We investigated the anti-carcinogenic effects of these isoprenols in HT-29 and HCT116 colon cancer cells and PPARγ involvement. Results indicate that the FOH- and GGOH-induced apoptosis involve caspase 3 activation, PARP cleavage, nuclear chromatin condensation, down-regulation of Bcl-xL and survivin expression, with increased PPARγ promoter activity. Pretreatment of the PPARγ antagonist GW9662 reduces FOH-induced growth inhibition and the associated PARP cleavage. We conclude that PPARγ activation is essential to elicit the anti-carcinogenic action of herbal isoprenols in colonic cancer cells.

AB - Farnesol (FOH) and geranylgeraniol (GGOH) possess anti-tumor potential, while peroxisome proliferator-activated receptor γ (PPARγ) has exhibited modulating effects in colorectal cancers. We investigated the anti-carcinogenic effects of these isoprenols in HT-29 and HCT116 colon cancer cells and PPARγ involvement. Results indicate that the FOH- and GGOH-induced apoptosis involve caspase 3 activation, PARP cleavage, nuclear chromatin condensation, down-regulation of Bcl-xL and survivin expression, with increased PPARγ promoter activity. Pretreatment of the PPARγ antagonist GW9662 reduces FOH-induced growth inhibition and the associated PARP cleavage. We conclude that PPARγ activation is essential to elicit the anti-carcinogenic action of herbal isoprenols in colonic cancer cells.

KW - FOH

KW - GGOH

KW - GW9662

KW - HCT116 cells

KW - HT-29 cells

KW - PPARγ

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U2 - 10.1080/07357900801898656

DO - 10.1080/07357900801898656

M3 - Journal article

C2 - 18608213

AN - SCOPUS:48249118758

SN - 0735-7907

VL - 26

SP - 708

EP - 717

JO - Cancer Investigation

JF - Cancer Investigation

IS - 7

ER -

Herbal isoprenols induce apoptosis in human colon cancer cells through transcriptional activation of PPARγ

Abstract

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UN SDGs

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