Growth characterisation of isolated indigenous iron-oxidising bacteria and their application in sludge treatment

L. Xiang, L. C. Chan, M. Fang, J. W. C. Wong

Research output: Chapter in book/report/conference proceedingConference proceeding


Indigenous iron-oxidising bacteria was acclimated and isolated from the sewage sludge originated from the Yuen Long wastewater treatment plant, Hong Kong. The acclimation process was performed by amending the sludge with FeSO47H2O at pH3.0 and 28°c for 23 days. The bacteria were isolated after several transfers in the improved leathen medium and were identified as Thiobacllus ferroxidans. The influence of pH, Fe2+ concentration and inoculum volume on the biooxidation of Fe2+ showed that the isolated bacteria had a strong ability in oxidizing Fe2+ under acidic condition. Following the inoculation of sludge with the indigenous iron-oxidising bacteria and the addition of FeSO47H2O, 5.9-53% of Cr. 81-90% of Cu, 78-83% of Zn, 27-54% of Pb and 0.7-16% of Ni could be leached from the sludge. In contrast, only 3.1% of Cr, 43% of Cu, 70% of Zn, 14% of Pb and 0.6% of Ni were removed in the control receiving no inoculation and Fe2+ addition. The present work confirmed the effectiveness of the isolated indigenous iron-oxidising bacteria for the removal heavy metals from sewage sludge.
Original languageEnglish
Title of host publicationAsian Waterqual '99
Subtitle of host publication7th IAWQ Asia-Pacific Regional Conference, Conference Preprint
PublisherInternational Association on Water Quality
Number of pages6
Publication statusPublished - Oct 1999
EventAsian Waterqual '99: 7th IAWQ Asia-Pacific Regional Conference - Taipei, Taiwan, Province of China
Duration: 18 Oct 199920 Oct 1999


ConferenceAsian Waterqual '99
Country/TerritoryTaiwan, Province of China

User-Defined Keywords

  • bioleaching
  • heavy metal
  • land application
  • sewage sludge
  • Thiobacillus ferrooxidans


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