GPER/Hippo-YAP signal is involved in Bisphenol S induced migration of triple negative breast cancer (TNBC) cells

Qianqian Deng; Guanmin Jiang; Yingmin Wu; Jiexin Li; Weiting Liang; Likun Chen; Qiao Su; Wuguo Li; Jun Du; Chris K. C. Wong; Zhuojia Chen; Hongsheng Wang

doi:10.1016/j.jhazmat.2018.05.013

GPER/Hippo-YAP signal is involved in Bisphenol S induced migration of triple negative breast cancer (TNBC) cells

Qianqian Deng
, Guanmin Jiang
, Yingmin Wu
, Jiexin Li
, Weiting Liang
, Likun Chen
, Qiao Su
, Wuguo Li
, Jun Du
, Chris K. C. Wong
, Zhuojia Chen^*
, Hongsheng Wang

^*Corresponding author for this work

Department of Biology

Research output: Contribution to journal › Journal article › peer-review

62 Citations (Scopus)

Abstract

Nowadays, risk factors of triple-negative breast cancer (TNBC) metastasis are not well identified. Our present study reveals that an industrial chemical, bisphenol S (BPS), can promote the migration, but not the proliferation, of TNBC cells in vitro. BPS activates YAP, a key effector of Hippo pathway, by inhibiting its phosphorylation, which promotes YAP nuclear accumulation and up-regulates its downstream genes such as CTGF and ANKRD1. Inhibition of YAP blocks the BPS-triggered cell migration and up-regulation of fibronectin (FN) and vimentin (Vim). BPS rapidly decreases the phosphorylation levels of LATS1 (Ser909) in TNBC cells, which regulates the activation and functions of YAP. Silencing LATS1/2 by siRNA increases BPS-induced dephosphorylation of YAP and extended the half-life of YAP protein. Inhibition of G protein-coupled estrogen receptor 1 (GPER) and its downstream PLCβ/PKC signals attenuate the effects of BPS-induced YAP dephosphorylation and CTGF up-regulation. Targeted inhibition of GPER/YAP inhibits BPS-induced migration of TNBC cells. Collectively, we reveal that GPER/Hippo-YAP signal is involved in BPS-induced migration of TNBC cells.

Original language	English
Pages (from-to)	1-9
Number of pages	9
Journal	Journal of Hazardous Materials
Volume	355
DOIs	https://doi.org/10.1016/j.jhazmat.2018.05.013
Publication status	Published - 5 Aug 2018

User-Defined Keywords

Bisphenol S
GPER
migration
TNBC
YAP

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.jhazmat.2018.05.013

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@article{aacd8040602c478092a1bfdb53f64f68,

title = "GPER/Hippo-YAP signal is involved in Bisphenol S induced migration of triple negative breast cancer (TNBC) cells",

abstract = "Nowadays, risk factors of triple-negative breast cancer (TNBC) metastasis are not well identified. Our present study reveals that an industrial chemical, bisphenol S (BPS), can promote the migration, but not the proliferation, of TNBC cells in vitro. BPS activates YAP, a key effector of Hippo pathway, by inhibiting its phosphorylation, which promotes YAP nuclear accumulation and up-regulates its downstream genes such as CTGF and ANKRD1. Inhibition of YAP blocks the BPS-triggered cell migration and up-regulation of fibronectin (FN) and vimentin (Vim). BPS rapidly decreases the phosphorylation levels of LATS1 (Ser909) in TNBC cells, which regulates the activation and functions of YAP. Silencing LATS1/2 by siRNA increases BPS-induced dephosphorylation of YAP and extended the half-life of YAP protein. Inhibition of G protein-coupled estrogen receptor 1 (GPER) and its downstream PLCβ/PKC signals attenuate the effects of BPS-induced YAP dephosphorylation and CTGF up-regulation. Targeted inhibition of GPER/YAP inhibits BPS-induced migration of TNBC cells. Collectively, we reveal that GPER/Hippo-YAP signal is involved in BPS-induced migration of TNBC cells.",

keywords = "Bisphenol S, GPER, migration, TNBC, YAP",

author = "Qianqian Deng and Guanmin Jiang and Yingmin Wu and Jiexin Li and Weiting Liang and Likun Chen and Qiao Su and Wuguo Li and Jun Du and Wong, \{Chris K. C.\} and Zhuojia Chen and Hongsheng Wang",

note = "Funding Information: This research was supported by the National Natural Science Foundation of China (Grant No. 81673454 , No. 81672608 , No. 81472470 , and No. 81572270 ), the Guangdong Natural Science Funds for Distinguished Young Scholar (No. 2014A030306025 ), the Pearl River S\&T Nova Program of Guangzhou (No. 1517000390 ), the Fundamental Research Funds for the Central Universities (Sun Yat-sen University) ( 16ykpy09 ), the Guangdong Province Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation (No. 2017B030314026 ), and the Science \& Technology Planning Project of Guangdong Province ( 2014A030304016 , 2017A030303015 ). Appendix A ",

year = "2018",

month = aug,

day = "5",

doi = "10.1016/j.jhazmat.2018.05.013",

language = "English",

volume = "355",

pages = "1--9",

journal = "Journal of Hazardous Materials",

issn = "0304-3894",

publisher = "Elsevier B.V.",

}

TY - JOUR

T1 - GPER/Hippo-YAP signal is involved in Bisphenol S induced migration of triple negative breast cancer (TNBC) cells

AU - Deng, Qianqian

AU - Jiang, Guanmin

AU - Wu, Yingmin

AU - Li, Jiexin

AU - Liang, Weiting

AU - Chen, Likun

AU - Su, Qiao

AU - Li, Wuguo

AU - Du, Jun

AU - Wong, Chris K. C.

AU - Chen, Zhuojia

AU - Wang, Hongsheng

N1 - Funding Information: This research was supported by the National Natural Science Foundation of China (Grant No. 81673454 , No. 81672608 , No. 81472470 , and No. 81572270 ), the Guangdong Natural Science Funds for Distinguished Young Scholar (No. 2014A030306025 ), the Pearl River S&T Nova Program of Guangzhou (No. 1517000390 ), the Fundamental Research Funds for the Central Universities (Sun Yat-sen University) ( 16ykpy09 ), the Guangdong Province Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation (No. 2017B030314026 ), and the Science & Technology Planning Project of Guangdong Province ( 2014A030304016 , 2017A030303015 ). Appendix A

PY - 2018/8/5

Y1 - 2018/8/5

N2 - Nowadays, risk factors of triple-negative breast cancer (TNBC) metastasis are not well identified. Our present study reveals that an industrial chemical, bisphenol S (BPS), can promote the migration, but not the proliferation, of TNBC cells in vitro. BPS activates YAP, a key effector of Hippo pathway, by inhibiting its phosphorylation, which promotes YAP nuclear accumulation and up-regulates its downstream genes such as CTGF and ANKRD1. Inhibition of YAP blocks the BPS-triggered cell migration and up-regulation of fibronectin (FN) and vimentin (Vim). BPS rapidly decreases the phosphorylation levels of LATS1 (Ser909) in TNBC cells, which regulates the activation and functions of YAP. Silencing LATS1/2 by siRNA increases BPS-induced dephosphorylation of YAP and extended the half-life of YAP protein. Inhibition of G protein-coupled estrogen receptor 1 (GPER) and its downstream PLCβ/PKC signals attenuate the effects of BPS-induced YAP dephosphorylation and CTGF up-regulation. Targeted inhibition of GPER/YAP inhibits BPS-induced migration of TNBC cells. Collectively, we reveal that GPER/Hippo-YAP signal is involved in BPS-induced migration of TNBC cells.

AB - Nowadays, risk factors of triple-negative breast cancer (TNBC) metastasis are not well identified. Our present study reveals that an industrial chemical, bisphenol S (BPS), can promote the migration, but not the proliferation, of TNBC cells in vitro. BPS activates YAP, a key effector of Hippo pathway, by inhibiting its phosphorylation, which promotes YAP nuclear accumulation and up-regulates its downstream genes such as CTGF and ANKRD1. Inhibition of YAP blocks the BPS-triggered cell migration and up-regulation of fibronectin (FN) and vimentin (Vim). BPS rapidly decreases the phosphorylation levels of LATS1 (Ser909) in TNBC cells, which regulates the activation and functions of YAP. Silencing LATS1/2 by siRNA increases BPS-induced dephosphorylation of YAP and extended the half-life of YAP protein. Inhibition of G protein-coupled estrogen receptor 1 (GPER) and its downstream PLCβ/PKC signals attenuate the effects of BPS-induced YAP dephosphorylation and CTGF up-regulation. Targeted inhibition of GPER/YAP inhibits BPS-induced migration of TNBC cells. Collectively, we reveal that GPER/Hippo-YAP signal is involved in BPS-induced migration of TNBC cells.

KW - Bisphenol S

KW - GPER

KW - migration

KW - TNBC

KW - YAP

UR - https://www.scopus.com/pages/publications/85046755478

U2 - 10.1016/j.jhazmat.2018.05.013

DO - 10.1016/j.jhazmat.2018.05.013

M3 - Journal article

C2 - 29758456

AN - SCOPUS:85046755478

SN - 0304-3894

VL - 355

SP - 1

EP - 9

JO - Journal of Hazardous Materials

JF - Journal of Hazardous Materials

ER -

GPER/Hippo-YAP signal is involved in Bisphenol S induced migration of triple negative breast cancer (TNBC) cells

Abstract

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