Glucuronide and Sulfate Conjugates of Bisphenol A: Chemical Synthesis and Correlation Between Their Urinary Levels and Plasma Bisphenol A Content in Voluntary Human Donors

Ka Lok Ho, Ka Ki Yuen, Man Shan Yau, Margaret B. Murphy, Yi Wan, Bonnie M.W. Fong, Sidney Tam, John P. Giesy, Kelvin S Y LEUNG, Michael H.W. Lam*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

Abstract: Bisphenol A (BPA) glucuronide and sulfate conjugates are major products of Phase II metabolism of BPA in humans. In the past, their determination in body fluids usually involves tedious enzymatic hydrolysis and multiresidual analysis. The recent availability of authentic standards of these conjugates enables our better understand of the human metabolism of BPA and the distribution of their metabolites in body fluids. In this work, we report the chemical synthesis and purification of BPA mono- and di-glucuronide and BPA mono- and di-sulfate. Their levels, as well as that of BPA, in 140 paired human plasma and urine samples collected randomly from voluntary donors in Hong Kong SAR, China, were determined by solid-phase extraction and liquid chromatography–tandem mass spectrometry (LC–MS/MS). BPA was found in more than 135 human plasma and urine samples. Its Phase II metabolites, ranging from N.D. to 36.7 µg g−1-creatinine, also were detected in 139 of the 140 urine samples. Good correlation (r = 0.911) between molar concentration of BPA in the plasma and that of “total urinary BPA” (i.e., ln [(BPA + ∑ BPA phase II conjugate)molar concentration]) was observed. Direct quantification of Phase II metabolites of BPA in human urine can be a useful assessment tool for population exposure to this potent endocrine disrupting chemical. Graphical Abstract: [Figure not available: see fulltext.].

Original languageEnglish
Pages (from-to)410-420
Number of pages11
JournalArchives of Environmental Contamination and Toxicology
Volume73
Issue number3
DOIs
Publication statusPublished - Oct 2017

Scopus Subject Areas

  • Toxicology
  • Pollution
  • Health, Toxicology and Mutagenesis

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