Abstract
A simple and relatively cheap glucose biosensor based on a combination of gold nanoparticles (Au NPs) and glucose oxidase (GOx) immobilized on a bioplatform eggshell membrane was established. Scanning electron microscopy showed successful immobilization of Au NPs/GOx on the eggshell membrane. The effects of pH, phosphate buffer concentration, and temperature on the glucose biosensor were studied in detail. The biosensor shows a linear response at a glucose concentration range of 5-525 μM. The detection limit of the biosensor is 2.5 μM (S/N = 3). The biosensor exhibits good repeatability with RSD = 3.6% (n = 6), good operational stability with over 300 measurements and long-term storage stability with a shelf life of at least 6 months. The response time is less than 60 s. The glucose level in commercial food samples has been successfully determined. The proposed work shows potential to develop cost-effective biosensors for biotechnological, biomedical and industrial use.
| Original language | English |
|---|---|
| Pages (from-to) | 492-500 |
| Number of pages | 9 |
| Journal | Biotechnology Journal |
| Volume | 6 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - May 2011 |
Scopus Subject Areas
- Applied Microbiology and Biotechnology
- Molecular Medicine
User-Defined Keywords
- Biosensors
- Eggshell membrane
- Enzymes
- Glucose oxidase
- Gold nanoparticles