Glucose biosensor based on nanohybrid material of gold nanoparticles and glucose oxidase on a bioplatform

Yan Zhang, Wenjuan Jia, Miao Cui, Chuan Dong, Shaomin Shuang*, Yuen Kwan, Martin M F CHOI

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

14 Citations (Scopus)


A simple and relatively cheap glucose biosensor based on a combination of gold nanoparticles (Au NPs) and glucose oxidase (GOx) immobilized on a bioplatform eggshell membrane was established. Scanning electron microscopy showed successful immobilization of Au NPs/GOx on the eggshell membrane. The effects of pH, phosphate buffer concentration, and temperature on the glucose biosensor were studied in detail. The biosensor shows a linear response at a glucose concentration range of 5-525 μM. The detection limit of the biosensor is 2.5 μM (S/N = 3). The biosensor exhibits good repeatability with RSD = 3.6% (n = 6), good operational stability with over 300 measurements and long-term storage stability with a shelf life of at least 6 months. The response time is less than 60 s. The glucose level in commercial food samples has been successfully determined. The proposed work shows potential to develop cost-effective biosensors for biotechnological, biomedical and industrial use.

Original languageEnglish
Pages (from-to)492-500
Number of pages9
JournalBiotechnology Journal
Issue number5
Publication statusPublished - May 2011

Scopus Subject Areas

  • Applied Microbiology and Biotechnology
  • Molecular Medicine

User-Defined Keywords

  • Biosensors
  • Eggshell membrane
  • Enzymes
  • Glucose oxidase
  • Gold nanoparticles


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