Glucocorticoid receptor is involved in the differential expression of hepatic 3β-hydroxysteroid dehydrogenase between barrows and boars at finishing stage

Xian Li, Rihua Cong, Wen Yao, Yimin Jia, Runsheng LI, Zhiyuan Sun, Xi Li, Ruqian Zhao*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The enzyme 3β-hydroxysteroid dehydrogenase (3β-HSD) plays an important role in androstenone metabolism in pig liver, and its defective expression is related to the development of boar taint. Early age castration is a common practice in many countries to avoid boar taint, yet whether and how castration affects porcine hepatic 3β-HSD expression are still poorly understood. In this study, we aimed to compare the expression of 3β-HSD between intact (boars) and castrated (barrows) male pigs, and to explore the potential factors regulating 3β-HSD transcription. Compared to barrows, boars showed worse carcass quality. Boars had significantly higher levels of serum androstenone (P < 0.01), testosterone (P < 0.01) and hepatic cortisol (P < 0.05), which were contrary to significantly lower expression of 3β-HSD messenger RNA (P < 0.01) and protein (P < 0.01) in the liver. Significant differences were detected for the hepatic expression of androgen receptor (AR) and CCAAT/enhancer binding protein β (C/EBPβ). Chromatin immunoprecipitation (ChIP) assay demonstrated reduced histone H3 acetylation (P < 0.05) but increased glucocorticoid receptor (GR) binding to 3β-HSD gene promoter in boars (P < 0.05). These results indicate that GR binding to 3β-HSD promoter is involved in the differential hepatic 3β-HSD expression between boars and barrows.

Original languageEnglish
Pages (from-to)158-166
Number of pages9
JournalAnimal Science Journal
Volume89
Issue number1
DOIs
Publication statusPublished - Jan 2018

Scopus Subject Areas

  • Agricultural and Biological Sciences(all)

User-Defined Keywords

  • 3β-HSD
  • androgen receptor
  • glucocorticoid receptor
  • liver
  • pig

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