Global Analysis of Dark- and Heat-Regulated Alternative Splicing in Arabidopsis

Di Zhang, Mo Xian Chen*, Mehtab Muhammad Aslam, Ying Gao Liu, Jianhua Zhang*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review


Alternative splicing (AS) is one of the major post-transcriptional regulation mechanisms that contributes to plant responses to various environmental perturbations. Darkness and heat are two common abiotic factors affecting plant growth, yet the involvement and regulation of AS in the plant responses to these signals remain insufficiently examined. In this study, we subjected Arabidopsis seedlings to 6 h of darkness or heat stress and analyzed their transcriptome through short-read RNA sequencing. We revealed that both treatments altered the transcription and AS of a subset of genes yet with different mechanisms. Dark-regulated AS events were found enriched in photosynthesis and light signaling pathways, while heat-regulated AS events were enriched in responses to abiotic stresses but not in heat-responsive genes, which responded primarily through transcriptional regulation. The AS of splicing-related genes (SRGs) was susceptible to both treatments; while dark treatment mostly regulated the AS of these genes, heat had a strong effect on both their transcription and AS. PCR analysis showed that the AS of the Serine/Arginine-rich family gene SR30 was reversely regulated by dark and heat, and heat induced the upregulation of multiple minor SR30 isoforms with intron retention. Our results suggest that AS participates in plant responses to these two abiotic signals and reveal the regulation of splicing regulators during these processes.

Original languageEnglish
Article number5299
Number of pages18
JournalInternational Journal of Molecular Sciences
Issue number6
Publication statusPublished - 2 Mar 2023

Scopus Subject Areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

User-Defined Keywords

  • abiotic stresses
  • alternative splicing
  • dark
  • heat stress
  • RNA sequencing
  • splicing factors
  • splicing-related genes
  • SR30


Dive into the research topics of 'Global Analysis of Dark- and Heat-Regulated Alternative Splicing in Arabidopsis'. Together they form a unique fingerprint.

Cite this