TY - JOUR
T1 - Genome-wide CRISPR–Cas9 Knockout Screening Reveals a TSPAN3-mediated Endo-lysosome Pathway Regulating the Degradation of α-Synuclein Oligomers
AU - Hu, Jun Jian
AU - Guan, Xinjie
AU - Zhao, Miao
AU - Xie, Pengqing
AU - Guo, Jifeng
AU - Tan, Jieqiong
N1 - Funding Information:
This work was supported by the National Key Research and Development Program of China (Grant No. 2021YFA0805200), National Natural Science Foundation of China (Grant No. 82171258), Changsha Science and Technology Foundation (Grant No. kq2004083), the Innovative Team Program 2019RS1010 from the Department of Science & Technology of Hunan Province, the Innovation-Driven Team Project 2020CX016 from Central South University, NHC Key Laboratory of Birth Defect for Research and Prevention, Hunan Provincial Maternal and Child Health Care Hospital, No. KF2021001. and Fundamental Research Funds for the Central Universities of Central South University (2022ZZTS0234, 2023ZZTS0254).
Publisher Copyright:
© The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2023
PY - 2023/11
Y1 - 2023/11
N2 - Misfolding and aggregation of α-Synuclein (α-Syn), which are hallmark pathological features of neurodegenerative diseases such as Parkinson’s disease (PD) and dementia with Lewy Bodies, continue to be significant areas of research. Among the diverse forms of α-Syn – monomer, oligomer, and fibril, the oligomer is considered the most toxic. However, the mechanisms governing α-Syn oligomerization are not yet fully understood. In this study, we utilized genome-wide CRISPR/Cas9 loss-of-function screening in human HEK293 cells to identify negative regulators of α-Syn oligomerization. We found that tetraspanin 3 (TSPAN3), a presumptive four-pass transmembrane protein, but not its homolog TSPAN7, significantly modulates α-Syn oligomer levels. TSPAN3 was observed to interact with α-Syn oligomers, regulate the amount of α-Syn oligomers on the cell membrane, and promote their degradation via the clathrin-AP2 mediated endo-lysosome pathway. Our findings highlight TSPAN3 as a potential regulator of α-Syn oligomers, presenting a promising target for future PD prevention and treatment strategies.
AB - Misfolding and aggregation of α-Synuclein (α-Syn), which are hallmark pathological features of neurodegenerative diseases such as Parkinson’s disease (PD) and dementia with Lewy Bodies, continue to be significant areas of research. Among the diverse forms of α-Syn – monomer, oligomer, and fibril, the oligomer is considered the most toxic. However, the mechanisms governing α-Syn oligomerization are not yet fully understood. In this study, we utilized genome-wide CRISPR/Cas9 loss-of-function screening in human HEK293 cells to identify negative regulators of α-Syn oligomerization. We found that tetraspanin 3 (TSPAN3), a presumptive four-pass transmembrane protein, but not its homolog TSPAN7, significantly modulates α-Syn oligomer levels. TSPAN3 was observed to interact with α-Syn oligomers, regulate the amount of α-Syn oligomers on the cell membrane, and promote their degradation via the clathrin-AP2 mediated endo-lysosome pathway. Our findings highlight TSPAN3 as a potential regulator of α-Syn oligomers, presenting a promising target for future PD prevention and treatment strategies.
KW - CRISPR-Cas9
KW - Degradation
KW - Lysosome
KW - TSPAN3
KW - α-Synuclein
UR - http://www.scopus.com/inward/record.url?scp=85165261611&partnerID=8YFLogxK
U2 - 10.1007/s12035-023-03495-5
DO - 10.1007/s12035-023-03495-5
M3 - Journal article
C2 - 37477766
AN - SCOPUS:85165261611
SN - 0893-7648
VL - 60
SP - 6731
EP - 6747
JO - Molecular Neurobiology
JF - Molecular Neurobiology
IS - 11
ER -
