TY - JOUR
T1 - Genome-centric polyhydroxyalkanoate reconciliation reveals nutrient enriched growth dependent biosynthesis in Bacillus cereus IBA1
AU - Kumar, Rajat
AU - Li, Dongyi
AU - Luo, Liwen
AU - Manu, M. K.
AU - Zhao, Jun
AU - Tyagi, Rajeshwar D.
AU - Wong, Jonathan W. C.
N1 - Funding Information:
We would like to thank the colleagues, editors, and anonymous reviewers for their constructive comments and suggestions. Additionally, the LABGeM (CEA/Genoscope & CNRS UMR8030), the France Génomique and French Bioinformatics Institute national infrastructures (funded as part of Investissement d'Avenir program managed by Agence Nationale pour la Recherche, contracts ANR-10-INBS-09 and ANR-11-INBS-0013) are acknowledged for support within the MicroScope annotation platform.
Copyright © 2023. Published by Elsevier Ltd.
PY - 2023/8
Y1 - 2023/8
N2 - Microbiological polyhydroxyalkanoates (PHAs) are rooted as the most promising bio-replacements of synthetic polymers. Inherent properties of these PHAs further expand their applicability in numerous industrial, environmental, and clinical sectors. To propel these, a new environmental, endotoxin free gram-positive bacterium i.e., Bacillus cereus IBA1 was identified to harbor advantageous PHA producer characteristics through high-throughput omics mining approaches. Unlike traditional fermentations, nutrient enriched strategy was used to enhance PHA granular concentrations by ∼2.3 folds to 2.78 ± 0.19 g/L. Additionally, this study is the first to confirm an underlying growth dependent PHA biogenesis through exploring PHA granule associated operons which harbour constitutively expressing PHA synthase (phaC) coupled with differentially expressing PHA synthase subunit (phaR) and regulatory protein (phaP, phaQ) amid different growth phases. Moreover, the feasibility of this promising microbial phenomenon could propel next-generation biopolymers, and increase industrial applicability of PHAs, thereby significantly contributing to the sustainable development.
AB - Microbiological polyhydroxyalkanoates (PHAs) are rooted as the most promising bio-replacements of synthetic polymers. Inherent properties of these PHAs further expand their applicability in numerous industrial, environmental, and clinical sectors. To propel these, a new environmental, endotoxin free gram-positive bacterium i.e., Bacillus cereus IBA1 was identified to harbor advantageous PHA producer characteristics through high-throughput omics mining approaches. Unlike traditional fermentations, nutrient enriched strategy was used to enhance PHA granular concentrations by ∼2.3 folds to 2.78 ± 0.19 g/L. Additionally, this study is the first to confirm an underlying growth dependent PHA biogenesis through exploring PHA granule associated operons which harbour constitutively expressing PHA synthase (phaC) coupled with differentially expressing PHA synthase subunit (phaR) and regulatory protein (phaP, phaQ) amid different growth phases. Moreover, the feasibility of this promising microbial phenomenon could propel next-generation biopolymers, and increase industrial applicability of PHAs, thereby significantly contributing to the sustainable development.
KW - Gram-positive
KW - Biopolymer
KW - Whole genome
KW - Nutrient conditioning
KW - PHA granule associated proteins
UR - http://www.scopus.com/inward/record.url?scp=85160028837&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2023.129210
DO - 10.1016/j.biortech.2023.129210
M3 - Journal article
C2 - 37217149
AN - SCOPUS:85160028837
SN - 0960-8524
VL - 382
JO - Bioresource Technology
JF - Bioresource Technology
M1 - 129210
ER -
