Gene expression profiling of human synovial sarcoma cell line (Hs701.T) in response to IL-1β stimulation

W. Y. Ha; X. J. Li; Patrick Y K YUE; D. Y.L. Wong; Kevin K M YUE; W. S. Chung; L. Zhao; P. Y. Leung; L. Liu; Ricky N S WONG

doi:10.1007/s00011-006-0086-9

Gene expression profiling of human synovial sarcoma cell line (Hs701.T) in response to IL-1β stimulation

W. Y. Ha, X. J. Li, Patrick Y K YUE, D. Y.L. Wong, Kevin K M YUE, W. S. Chung, L. Zhao, P. Y. Leung, L. Liu, Ricky N S WONG^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › peer-review

7 Citations (Scopus)

Abstract

Objective: Synovial sarcoma (SS) is a malignant mesenchymal tumor that accounts for 5-10% of all soft tissue sarcoma. IL-1β, a pleiotrophic cytokine, has been found in the tumor microenvironment which plays crucial roles in the pathogenesis of tumors. Methods: In this study, we used Hs701.T as a cellular model to study the short-term (4-h) and long-term (48-h) stimulatory effect of IL-1β on cell proliferation and differential gene expression. Results: The results showed that IL-1β can stimulate cell proliferation through activation of NF-κB and AP-1 transcription factors; sequentially triggers the expression of genes related to tumor progression. The microarray data indicated that most of the up-regulated genes were related to tumor progression. Five candidate genes which are involved in the mediation of proliferation (IL-6), apoptosis (Hsp27 and Daxx), and angiogenesis (PlGF and SPARC) were further validated by RT-PCR. Conclusion: These findings may be useful for understanding the pathogenesis of synovial sarcoma.

Original language	English
Pages (from-to)	293-299
Number of pages	7
Journal	Inflammation Research
Volume	55
Issue number	7
DOIs	https://doi.org/10.1007/s00011-006-0086-9
Publication status	Published - Jul 2006

Scopus Subject Areas

Immunology
Pharmacology

User-Defined Keywords

DNA Microarray
Hs701.T cells
Human synovial sarcoma
IL-1β
Tumor progression

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10.1007/s00011-006-0086-9

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@article{fc2557e234134cd9bb19d5bae4c3ffed,

title = "Gene expression profiling of human synovial sarcoma cell line (Hs701.T) in response to IL-1β stimulation",

abstract = "Objective: Synovial sarcoma (SS) is a malignant mesenchymal tumor that accounts for 5-10% of all soft tissue sarcoma. IL-1β, a pleiotrophic cytokine, has been found in the tumor microenvironment which plays crucial roles in the pathogenesis of tumors. Methods: In this study, we used Hs701.T as a cellular model to study the short-term (4-h) and long-term (48-h) stimulatory effect of IL-1β on cell proliferation and differential gene expression. Results: The results showed that IL-1β can stimulate cell proliferation through activation of NF-κB and AP-1 transcription factors; sequentially triggers the expression of genes related to tumor progression. The microarray data indicated that most of the up-regulated genes were related to tumor progression. Five candidate genes which are involved in the mediation of proliferation (IL-6), apoptosis (Hsp27 and Daxx), and angiogenesis (PlGF and SPARC) were further validated by RT-PCR. Conclusion: These findings may be useful for understanding the pathogenesis of synovial sarcoma.",

keywords = "DNA Microarray, Hs701.T cells, Human synovial sarcoma, IL-1β, Tumor progression",

author = "Ha, {W. Y.} and Li, {X. J.} and YUE, {Patrick Y K} and Wong, {D. Y.L.} and YUE, {Kevin K M} and Chung, {W. S.} and L. Zhao and Leung, {P. Y.} and L. Liu and WONG, {Ricky N S}",

note = "Funding Information: Acknowledgements. This work was supported by the Earmark Research Funding Information: Grants (RGC Ref no. HKBU 2144/03M) of the Research Grant Committee, Hong Kong SAR Government.",

year = "2006",

month = jul,

doi = "10.1007/s00011-006-0086-9",

language = "English",

volume = "55",

pages = "293--299",

journal = "Inflammation Research",

issn = "1023-3830",

publisher = "Birkhauser Verlag Basel",

number = "7",

}

TY - JOUR

T1 - Gene expression profiling of human synovial sarcoma cell line (Hs701.T) in response to IL-1β stimulation

AU - Ha, W. Y.

AU - Li, X. J.

AU - YUE, Patrick Y K

AU - Wong, D. Y.L.

AU - YUE, Kevin K M

AU - Chung, W. S.

AU - Zhao, L.

AU - Leung, P. Y.

AU - Liu, L.

AU - WONG, Ricky N S

N1 - Funding Information: Acknowledgements. This work was supported by the Earmark Research Funding Information: Grants (RGC Ref no. HKBU 2144/03M) of the Research Grant Committee, Hong Kong SAR Government.

PY - 2006/7

Y1 - 2006/7

N2 - Objective: Synovial sarcoma (SS) is a malignant mesenchymal tumor that accounts for 5-10% of all soft tissue sarcoma. IL-1β, a pleiotrophic cytokine, has been found in the tumor microenvironment which plays crucial roles in the pathogenesis of tumors. Methods: In this study, we used Hs701.T as a cellular model to study the short-term (4-h) and long-term (48-h) stimulatory effect of IL-1β on cell proliferation and differential gene expression. Results: The results showed that IL-1β can stimulate cell proliferation through activation of NF-κB and AP-1 transcription factors; sequentially triggers the expression of genes related to tumor progression. The microarray data indicated that most of the up-regulated genes were related to tumor progression. Five candidate genes which are involved in the mediation of proliferation (IL-6), apoptosis (Hsp27 and Daxx), and angiogenesis (PlGF and SPARC) were further validated by RT-PCR. Conclusion: These findings may be useful for understanding the pathogenesis of synovial sarcoma.

AB - Objective: Synovial sarcoma (SS) is a malignant mesenchymal tumor that accounts for 5-10% of all soft tissue sarcoma. IL-1β, a pleiotrophic cytokine, has been found in the tumor microenvironment which plays crucial roles in the pathogenesis of tumors. Methods: In this study, we used Hs701.T as a cellular model to study the short-term (4-h) and long-term (48-h) stimulatory effect of IL-1β on cell proliferation and differential gene expression. Results: The results showed that IL-1β can stimulate cell proliferation through activation of NF-κB and AP-1 transcription factors; sequentially triggers the expression of genes related to tumor progression. The microarray data indicated that most of the up-regulated genes were related to tumor progression. Five candidate genes which are involved in the mediation of proliferation (IL-6), apoptosis (Hsp27 and Daxx), and angiogenesis (PlGF and SPARC) were further validated by RT-PCR. Conclusion: These findings may be useful for understanding the pathogenesis of synovial sarcoma.

KW - DNA Microarray

KW - Hs701.T cells

KW - Human synovial sarcoma

KW - IL-1β

KW - Tumor progression

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U2 - 10.1007/s00011-006-0086-9

DO - 10.1007/s00011-006-0086-9

M3 - Journal article

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SN - 1023-3830

VL - 55

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JO - Inflammation Research

JF - Inflammation Research

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ER -

Gene expression profiling of human synovial sarcoma cell line (Hs701.T) in response to IL-1β stimulation

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