Gene expression profiling of human synovial sarcoma cell line (Hs701.T) in response to IL-1β stimulation

W. Y. Ha, X. J. Li, Patrick Y K YUE, D. Y.L. Wong, Kevin K M YUE, W. S. Chung, L. Zhao, P. Y. Leung, L. Liu, Ricky N S WONG*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

7 Citations (Scopus)


Objective: Synovial sarcoma (SS) is a malignant mesenchymal tumor that accounts for 5-10% of all soft tissue sarcoma. IL-1β, a pleiotrophic cytokine, has been found in the tumor microenvironment which plays crucial roles in the pathogenesis of tumors. Methods: In this study, we used Hs701.T as a cellular model to study the short-term (4-h) and long-term (48-h) stimulatory effect of IL-1β on cell proliferation and differential gene expression. Results: The results showed that IL-1β can stimulate cell proliferation through activation of NF-κB and AP-1 transcription factors; sequentially triggers the expression of genes related to tumor progression. The microarray data indicated that most of the up-regulated genes were related to tumor progression. Five candidate genes which are involved in the mediation of proliferation (IL-6), apoptosis (Hsp27 and Daxx), and angiogenesis (PlGF and SPARC) were further validated by RT-PCR. Conclusion: These findings may be useful for understanding the pathogenesis of synovial sarcoma.

Original languageEnglish
Pages (from-to)293-299
Number of pages7
JournalInflammation Research
Issue number7
Publication statusPublished - Jul 2006

Scopus Subject Areas

  • Immunology
  • Pharmacology

User-Defined Keywords

  • DNA Microarray
  • Hs701.T cells
  • Human synovial sarcoma
  • IL-1β
  • Tumor progression


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