Gas chromatography-mass spectrometric determination of total isothiocyanates in Chinese medicinal herbs

Martin M.F. Choi; Shaomin Shuang; H. Y. Lai; S. C. Cheng; Rebecca C.W. Cheng; Bohr K.B. Cheung; Albert W.M. Lee

doi:10.1016/j.aca.2004.04.010

Gas chromatography-mass spectrometric determination of total isothiocyanates in Chinese medicinal herbs

Martin M.F. Choi^*, Shaomin Shuang, H. Y. Lai, S. C. Cheng, Rebecca C.W. Cheng, Bohr K.B. Cheung, Albert W.M. Lee

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

23 Citations (Scopus)

Abstract

A gas chromatography-mass spectrometric (GC-MS) method for the determination of total isothiocyanates (ITCs) has been developed based on the quantification of the cyclocondensation product, 1,3-benzodithiole-2-thione, between ITCs and 1,2-benzenedithiol. It was the first analytical application to be applied to the analysis of Chinese medicinal herbs including Laifuzi, Baijiezi, Tinglizi and Hancai, and seeds of commonly consumed vegetables. A considerable variation in ITC contents was observed. The highest concentrations of total ITCs were found to be 20.3 μmol/g in Laifuzi and 12.6 μmol/g in seeds of Chinese kale. The method demonstrates the usefulness of the cyclocondensation of ITCs with 1,2-benzenedithiol reagent for quantifying total ITC contents in Chinese medicinal herbs regardless of the types of ITC. The linear dynamic range covered from 0.12 to 200 μM ITC. The limit of detection (3S_b) was 35 nM and the corresponding absolute detection limit was 35 fmol. The proposed GC-MS technique provides a reliable, unambiguous, reproducible, and efficient method for determining total ITCs of various samples.

Original language	English
Pages (from-to)	155-163
Number of pages	9
Journal	Analytica Chimica Acta
Volume	516
Issue number	1-2
DOIs	https://doi.org/10.1016/j.aca.2004.04.010
Publication status	Published - 19 Jul 2004

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Chinese medicinal herbs
Gas chromatography-mass spectrometry
Isothiocyanates

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@article{a1aa09d8a3624bd497161f3175858573,

title = "Gas chromatography-mass spectrometric determination of total isothiocyanates in Chinese medicinal herbs",

abstract = "A gas chromatography-mass spectrometric (GC-MS) method for the determination of total isothiocyanates (ITCs) has been developed based on the quantification of the cyclocondensation product, 1,3-benzodithiole-2-thione, between ITCs and 1,2-benzenedithiol. It was the first analytical application to be applied to the analysis of Chinese medicinal herbs including Laifuzi, Baijiezi, Tinglizi and Hancai, and seeds of commonly consumed vegetables. A considerable variation in ITC contents was observed. The highest concentrations of total ITCs were found to be 20.3 μmol/g in Laifuzi and 12.6 μmol/g in seeds of Chinese kale. The method demonstrates the usefulness of the cyclocondensation of ITCs with 1,2-benzenedithiol reagent for quantifying total ITC contents in Chinese medicinal herbs regardless of the types of ITC. The linear dynamic range covered from 0.12 to 200 μM ITC. The limit of detection (3Sb) was 35 nM and the corresponding absolute detection limit was 35 fmol. The proposed GC-MS technique provides a reliable, unambiguous, reproducible, and efficient method for determining total ITCs of various samples.",

keywords = "Chinese medicinal herbs, Gas chromatography-mass spectrometry, Isothiocyanates",

author = "Choi, {Martin M.F.} and Shaomin Shuang and Lai, {H. Y.} and Cheng, {S. C.} and Cheng, {Rebecca C.W.} and Cheung, {Bohr K.B.} and Lee, {Albert W.M.}",

note = "Funding Information: Financial support of this research from HKBU (project No. FRG/01-02/II-22) is gratefully acknowledged.",

year = "2004",

month = jul,

day = "19",

doi = "10.1016/j.aca.2004.04.010",

language = "English",

volume = "516",

pages = "155--163",

journal = "Analytica Chimica Acta",

issn = "0003-2670",

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TY - JOUR

T1 - Gas chromatography-mass spectrometric determination of total isothiocyanates in Chinese medicinal herbs

AU - Choi, Martin M.F.

AU - Shuang, Shaomin

AU - Lai, H. Y.

AU - Cheng, S. C.

AU - Cheng, Rebecca C.W.

AU - Cheung, Bohr K.B.

AU - Lee, Albert W.M.

N1 - Funding Information: Financial support of this research from HKBU (project No. FRG/01-02/II-22) is gratefully acknowledged.

PY - 2004/7/19

Y1 - 2004/7/19

N2 - A gas chromatography-mass spectrometric (GC-MS) method for the determination of total isothiocyanates (ITCs) has been developed based on the quantification of the cyclocondensation product, 1,3-benzodithiole-2-thione, between ITCs and 1,2-benzenedithiol. It was the first analytical application to be applied to the analysis of Chinese medicinal herbs including Laifuzi, Baijiezi, Tinglizi and Hancai, and seeds of commonly consumed vegetables. A considerable variation in ITC contents was observed. The highest concentrations of total ITCs were found to be 20.3 μmol/g in Laifuzi and 12.6 μmol/g in seeds of Chinese kale. The method demonstrates the usefulness of the cyclocondensation of ITCs with 1,2-benzenedithiol reagent for quantifying total ITC contents in Chinese medicinal herbs regardless of the types of ITC. The linear dynamic range covered from 0.12 to 200 μM ITC. The limit of detection (3Sb) was 35 nM and the corresponding absolute detection limit was 35 fmol. The proposed GC-MS technique provides a reliable, unambiguous, reproducible, and efficient method for determining total ITCs of various samples.

AB - A gas chromatography-mass spectrometric (GC-MS) method for the determination of total isothiocyanates (ITCs) has been developed based on the quantification of the cyclocondensation product, 1,3-benzodithiole-2-thione, between ITCs and 1,2-benzenedithiol. It was the first analytical application to be applied to the analysis of Chinese medicinal herbs including Laifuzi, Baijiezi, Tinglizi and Hancai, and seeds of commonly consumed vegetables. A considerable variation in ITC contents was observed. The highest concentrations of total ITCs were found to be 20.3 μmol/g in Laifuzi and 12.6 μmol/g in seeds of Chinese kale. The method demonstrates the usefulness of the cyclocondensation of ITCs with 1,2-benzenedithiol reagent for quantifying total ITC contents in Chinese medicinal herbs regardless of the types of ITC. The linear dynamic range covered from 0.12 to 200 μM ITC. The limit of detection (3Sb) was 35 nM and the corresponding absolute detection limit was 35 fmol. The proposed GC-MS technique provides a reliable, unambiguous, reproducible, and efficient method for determining total ITCs of various samples.

KW - Chinese medicinal herbs

KW - Gas chromatography-mass spectrometry

KW - Isothiocyanates

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U2 - 10.1016/j.aca.2004.04.010

DO - 10.1016/j.aca.2004.04.010

M3 - Journal article

AN - SCOPUS:3042523469

SN - 0003-2670

VL - 516

SP - 155

EP - 163

JO - Analytica Chimica Acta

JF - Analytica Chimica Acta

IS - 1-2

ER -

Gas chromatography-mass spectrometric determination of total isothiocyanates in Chinese medicinal herbs

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