TY - JOUR
T1 - Gas chromatography-mass spectrometric determination of total isothiocyanates in Chinese medicinal herbs
AU - CHOI, Martin M F
AU - Shuang, Shaomin
AU - Lai, H. Y.
AU - Cheng, S. C.
AU - Cheng, Rebecca C.W.
AU - Cheung, Bohr K.B.
AU - LEE, Albert W M
N1 - Funding Information:
Financial support of this research from HKBU (project No. FRG/01-02/II-22) is gratefully acknowledged.
PY - 2004/7/19
Y1 - 2004/7/19
N2 - A gas chromatography-mass spectrometric (GC-MS) method for the determination of total isothiocyanates (ITCs) has been developed based on the quantification of the cyclocondensation product, 1,3-benzodithiole-2-thione, between ITCs and 1,2-benzenedithiol. It was the first analytical application to be applied to the analysis of Chinese medicinal herbs including Laifuzi, Baijiezi, Tinglizi and Hancai, and seeds of commonly consumed vegetables. A considerable variation in ITC contents was observed. The highest concentrations of total ITCs were found to be 20.3 μmol/g in Laifuzi and 12.6 μmol/g in seeds of Chinese kale. The method demonstrates the usefulness of the cyclocondensation of ITCs with 1,2-benzenedithiol reagent for quantifying total ITC contents in Chinese medicinal herbs regardless of the types of ITC. The linear dynamic range covered from 0.12 to 200 μM ITC. The limit of detection (3Sb) was 35 nM and the corresponding absolute detection limit was 35 fmol. The proposed GC-MS technique provides a reliable, unambiguous, reproducible, and efficient method for determining total ITCs of various samples.
AB - A gas chromatography-mass spectrometric (GC-MS) method for the determination of total isothiocyanates (ITCs) has been developed based on the quantification of the cyclocondensation product, 1,3-benzodithiole-2-thione, between ITCs and 1,2-benzenedithiol. It was the first analytical application to be applied to the analysis of Chinese medicinal herbs including Laifuzi, Baijiezi, Tinglizi and Hancai, and seeds of commonly consumed vegetables. A considerable variation in ITC contents was observed. The highest concentrations of total ITCs were found to be 20.3 μmol/g in Laifuzi and 12.6 μmol/g in seeds of Chinese kale. The method demonstrates the usefulness of the cyclocondensation of ITCs with 1,2-benzenedithiol reagent for quantifying total ITC contents in Chinese medicinal herbs regardless of the types of ITC. The linear dynamic range covered from 0.12 to 200 μM ITC. The limit of detection (3Sb) was 35 nM and the corresponding absolute detection limit was 35 fmol. The proposed GC-MS technique provides a reliable, unambiguous, reproducible, and efficient method for determining total ITCs of various samples.
KW - Chinese medicinal herbs
KW - Gas chromatography-mass spectrometry
KW - Isothiocyanates
UR - http://www.scopus.com/inward/record.url?scp=3042523469&partnerID=8YFLogxK
U2 - 10.1016/j.aca.2004.04.010
DO - 10.1016/j.aca.2004.04.010
M3 - Article
AN - SCOPUS:3042523469
VL - 516
SP - 155
EP - 163
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
SN - 0003-2670
IS - 1-2
ER -