TY - JOUR
T1 - G-quadruplex-based detection of glyphosate in complex biological systems by a time-resolved luminescent assay
AU - Chen, Feng
AU - Li, Guodong
AU - Liu, Hao
AU - Leung, Chung Hang
AU - Ma, Edmond Dik Lung
N1 - Funding Information:
This work was supported by Hong Kong Baptist University ( FRG2/17-18/003 ), the Health and Medical Research Fund ( HMRF/14150561 ), the National Natural Science Foundation of China ( 21575121 and 21775131 ), the Hong Kong Baptist University Century Club Sponsorship Scheme 2018 , the Interdisciplinary Research Matching Scheme ( RC-IRMS/16-17/03 ), Interdisciplinary Research Clusters Matching Scheme ( RC-IRCs/17-18/03 ), Collaborative Research Fund ( C5026-16G ), SKLEBA and HKBU Strategic Development Fund ( SKLP_1920_P02 ), The Science and Technology Development Fund , Macau SAR (File no. 0016/2020/A), and the University of Macau ( MYRG2018-00187-ICMS and MYRG2019-00002-ICMS ).
PY - 2020/10/1
Y1 - 2020/10/1
N2 - A luminescent G-quadruplex-derived detection technique was established to monitor glyphosate (GLY) using a phosphorescent iridium(III)-based probe. By exploiting the long lifetime of the probe, the use of time-resolved emission spectroscopy (TRES) could overcome substantial fluorescence background interference. This platform displayed excellent sensitivity for GLY with a limit of detection of 26.4 nM in Tris buffer, and 79.3 nM in Tris buffer containing soybean extract to simulate a complex environment. Due to the specificity of the GLY aptamer, the platform could selectively recognize GLY in complex samples. The feasibility of the method was evaluated by analysis of licorice, ginseng, ginger, and soybean samples, as well as in HUVEC and LO2 cell extracts, with good recovery values ranging from 85 % to 110 %. Importantly, GLY excreted by mouse fed with GLY could also be rapidly and efficiently detected with high correlation with a commercial kit, indicating the reliability of this platform for the rapid and efficient monitoring GLY in animals exposed to GLY. Finally, besides being a rapid and efficient detection method, this method also has the potential for rapid diagnosis at the point of care.
AB - A luminescent G-quadruplex-derived detection technique was established to monitor glyphosate (GLY) using a phosphorescent iridium(III)-based probe. By exploiting the long lifetime of the probe, the use of time-resolved emission spectroscopy (TRES) could overcome substantial fluorescence background interference. This platform displayed excellent sensitivity for GLY with a limit of detection of 26.4 nM in Tris buffer, and 79.3 nM in Tris buffer containing soybean extract to simulate a complex environment. Due to the specificity of the GLY aptamer, the platform could selectively recognize GLY in complex samples. The feasibility of the method was evaluated by analysis of licorice, ginseng, ginger, and soybean samples, as well as in HUVEC and LO2 cell extracts, with good recovery values ranging from 85 % to 110 %. Importantly, GLY excreted by mouse fed with GLY could also be rapidly and efficiently detected with high correlation with a commercial kit, indicating the reliability of this platform for the rapid and efficient monitoring GLY in animals exposed to GLY. Finally, besides being a rapid and efficient detection method, this method also has the potential for rapid diagnosis at the point of care.
KW - G-quadruplex
KW - Glyphosate
KW - Iridium(III) complex probe
KW - Time-Resolved emission spectroscopy
UR - http://www.scopus.com/inward/record.url?scp=85085984927&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2020.128393
DO - 10.1016/j.snb.2020.128393
M3 - Journal article
AN - SCOPUS:85085984927
SN - 0925-4005
VL - 320
JO - Sensors and Actuators B: Chemical
JF - Sensors and Actuators B: Chemical
M1 - 128393
ER -