Functional phosphoproteomic analysis reveals that a serine-62-phosphorylated isoform of ethylene response factor110 is involved in arabidopsis bolting

Lin Zhu, Dandan Liu, Yaojun Li, Ning Li*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

Ethylene is a major plant hormone that plays an important role in regulating bolting, although the underlying molecular mechanism is not well understood. In this study, we report the novel finding that the serine-62 (Ser-62) phosphorylation of Ethylene Response Factor110 (ERF110) is involved in the regulation of bolting time. The gene expression and posttranslational modification (phosphorylation) of ERF110 were analyzed among ethylene-response mutants and ERF110 RNA-interfering knockout lines of Arabidopsis (Arabidopsis thaliana). Physiological and biochemical studies revealed that the Ser-62 phosphorylation of ERF110 was closely related to bolting time, that is, the ethylene-enhanced gene expression of ERF110 and the decreased Ser-62 phosphorylation of the ERF110 protein in Arabidopsis. The expression of a flowering homeotic APETALA1 gene was upregulated by the Ser-62-phosphorylated isoform of the ERF110 transcription factor, which was necessary but not sufficient for normal bolting. The gene expression and phosphorylation of ERF110 were regulated by ethylene via both Ethylene- Insensitive2-dependent and -independent pathways, which constitute a dual-and-opposing mechanism of action for ethylene in the regulation of Arabidopsis bolting.

Original languageEnglish
Pages (from-to)904-917
Number of pages14
JournalPlant Physiology
Volume161
Issue number2
Early online date27 Nov 2012
DOIs
Publication statusPublished - Feb 2013

Scopus Subject Areas

  • Physiology
  • Genetics
  • Plant Science

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