FRET-based modified graphene quantum dots for direct trypsin quantification in urine

Chung Yan Poon, Qinghua Li, Jiali Zhang, Zhongping Li, Chuan Dong, Albert W M LEE, Wing Hong CHAN, Hung Wing LI*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

45 Citations (Scopus)

Abstract

A versatile nanoprobe was developed for trypsin quantification with fluorescence resonance energy transfer (FRET). Here, fluorescence graphene quantum dot is utilized as a donor while a well-designed coumarin derivative, CMR2, as an acceptor. Moreover, bovine serum albumin (BSA), as a protein model, is not only served as a linker for the FRET pair, but also a fluorescence enhancer of the quantum dots and CMR2. In the presence of trypsin, the FRET system would be destroyed when the BSA is digested by trypsin. Thus, the emission peak of the donor is regenerated and the ratio of emission peak of donor/emission peak of acceptor increased. By the ratiometric measurement of these two emission peaks, trypsin content could be determined. The detection limit of trypsin was found to be 0.7 μg/mL, which is 0.008-fold of the average trypsin level in acute pancreatitis patient's urine suggesting a high potential for fast and low cost clinical screening.

Original languageEnglish
Pages (from-to)64-70
Number of pages7
JournalAnalytica Chimica Acta
Volume917
DOIs
Publication statusPublished - 21 Apr 2016

Scopus Subject Areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

User-Defined Keywords

  • BSA
  • Coumarin
  • FRET
  • GQD
  • Trypsin

Fingerprint

Dive into the research topics of 'FRET-based modified graphene quantum dots for direct trypsin quantification in urine'. Together they form a unique fingerprint.

Cite this