Fractionation and characterization of sludge bacterial extracellular polymers by FT-IR, 13C-NMR, 1H-NMR

L. X. Zhou; J. R. Liang; X. H. Zhan; J. W.C. Wong

doi:10.2166/wst.2001.0584

Fractionation and characterization of sludge bacterial extracellular polymers by FT-IR, ¹³C-NMR, ¹H-NMR

L. X. Zhou^*, J. R. Liang, X. H. Zhan, J. W.C. Wong

^*Corresponding author for this work

Department of Biology

Research output: Contribution to journal › Journal article › peer-review

10 Citations (Scopus)

Abstract

Sludge bacterial extracellular (BEP) polymers exhibit a strong affinity with metals in addition to their role in the bioflocculation of activated sludge. It is of utmost importance in elucidating the chemical characterization of sludge BEP prior to a better understanding of its environmental behavior. Sludge BEP extracted from activated sludge was characterized by FT-IR, ¹H-NMR, and ¹³C-NMR and fractionated into 6 fractions (hydrophilic acid, base, and neutral; and hydrophobic acid, base, and neutral). The results indicated that sludge BEP contained more polysaccharides but fewer amino acids or proteinaceous materials compared to dissolved organic matter (DOM) derived from sludge. No aromatic substance was found in sludge BEP as indicated by NMR. Sludge BEP was dominated by hydrophilic fractions, accounting for 65% of total DOC of sludge BEP and hydrophilic neutral constituted about 58% of the hydrophilic fraction. The chemical fraction and characterization indicated that sludge BEP might exhibit a higher capability in enhancing the transport of the associated contaminants through porous media than that of sludge DOM.

Original language	English
Pages (from-to)	71-78
Number of pages	8
Journal	Water Science and Technology
Volume	44
Issue number	10
DOIs	https://doi.org/10.2166/wst.2001.0584
Publication status	Published - 2001

Scopus Subject Areas

Environmental Engineering
Water Science and Technology

User-Defined Keywords

Bacterial extracellular polymers
Hydrophilic
Hydrophobic
Sewage sludge
Spectroscopy

Access to Document

10.2166/wst.2001.0584

Cite this

@article{d4b4f9f386fe42b9ba6d1ffbe38e2b61,

title = "Fractionation and characterization of sludge bacterial extracellular polymers by FT-IR, 13C-NMR, 1H-NMR",

abstract = "Sludge bacterial extracellular (BEP) polymers exhibit a strong affinity with metals in addition to their role in the bioflocculation of activated sludge. It is of utmost importance in elucidating the chemical characterization of sludge BEP prior to a better understanding of its environmental behavior. Sludge BEP extracted from activated sludge was characterized by FT-IR, 1H-NMR, and 13C-NMR and fractionated into 6 fractions (hydrophilic acid, base, and neutral; and hydrophobic acid, base, and neutral). The results indicated that sludge BEP contained more polysaccharides but fewer amino acids or proteinaceous materials compared to dissolved organic matter (DOM) derived from sludge. No aromatic substance was found in sludge BEP as indicated by NMR. Sludge BEP was dominated by hydrophilic fractions, accounting for 65% of total DOC of sludge BEP and hydrophilic neutral constituted about 58% of the hydrophilic fraction. The chemical fraction and characterization indicated that sludge BEP might exhibit a higher capability in enhancing the transport of the associated contaminants through porous media than that of sludge DOM.",

keywords = "Bacterial extracellular polymers, Hydrophilic, Hydrophobic, Sewage sludge, Spectroscopy",

author = "Zhou, {L. X.} and Liang, {J. R.} and Zhan, {X. H.} and Wong, {J. W.C.}",

year = "2001",

doi = "10.2166/wst.2001.0584",

language = "English",

volume = "44",

pages = "71--78",

journal = "Water Science and Technology",

issn = "0273-1223",

publisher = "IWA Publishing",

number = "10",

}

TY - JOUR

T1 - Fractionation and characterization of sludge bacterial extracellular polymers by FT-IR, 13C-NMR, 1H-NMR

AU - Zhou, L. X.

AU - Liang, J. R.

AU - Zhan, X. H.

AU - Wong, J. W.C.

PY - 2001

Y1 - 2001

N2 - Sludge bacterial extracellular (BEP) polymers exhibit a strong affinity with metals in addition to their role in the bioflocculation of activated sludge. It is of utmost importance in elucidating the chemical characterization of sludge BEP prior to a better understanding of its environmental behavior. Sludge BEP extracted from activated sludge was characterized by FT-IR, 1H-NMR, and 13C-NMR and fractionated into 6 fractions (hydrophilic acid, base, and neutral; and hydrophobic acid, base, and neutral). The results indicated that sludge BEP contained more polysaccharides but fewer amino acids or proteinaceous materials compared to dissolved organic matter (DOM) derived from sludge. No aromatic substance was found in sludge BEP as indicated by NMR. Sludge BEP was dominated by hydrophilic fractions, accounting for 65% of total DOC of sludge BEP and hydrophilic neutral constituted about 58% of the hydrophilic fraction. The chemical fraction and characterization indicated that sludge BEP might exhibit a higher capability in enhancing the transport of the associated contaminants through porous media than that of sludge DOM.

AB - Sludge bacterial extracellular (BEP) polymers exhibit a strong affinity with metals in addition to their role in the bioflocculation of activated sludge. It is of utmost importance in elucidating the chemical characterization of sludge BEP prior to a better understanding of its environmental behavior. Sludge BEP extracted from activated sludge was characterized by FT-IR, 1H-NMR, and 13C-NMR and fractionated into 6 fractions (hydrophilic acid, base, and neutral; and hydrophobic acid, base, and neutral). The results indicated that sludge BEP contained more polysaccharides but fewer amino acids or proteinaceous materials compared to dissolved organic matter (DOM) derived from sludge. No aromatic substance was found in sludge BEP as indicated by NMR. Sludge BEP was dominated by hydrophilic fractions, accounting for 65% of total DOC of sludge BEP and hydrophilic neutral constituted about 58% of the hydrophilic fraction. The chemical fraction and characterization indicated that sludge BEP might exhibit a higher capability in enhancing the transport of the associated contaminants through porous media than that of sludge DOM.

KW - Bacterial extracellular polymers

KW - Hydrophilic

KW - Hydrophobic

KW - Sewage sludge

KW - Spectroscopy

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