Fluorescence turn-on probes for intracellular RNA distribution and their imaging in confocal and two-photon fluorescence microscopy

Guofen Song; Fang Miao; Yuming Sun; Xiaoqiang Yu; Jing Zhi Sun; Wai Yeung Wong

doi:10.1016/j.snb.2012.07.023

Fluorescence turn-on probes for intracellular RNA distribution and their imaging in confocal and two-photon fluorescence microscopy

Guofen Song
, Fang Miao
, Yuming Sun
, Xiaoqiang Yu^*
, Jing Zhi Sun
, Wai Yeung Wong

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

26 Citations (Scopus)

Abstract

We have synthesized four indole derivatives (INS1, IND1, INS2 and IND2) successfully, and experimental results of biochemical RNA fluorescence assays and direct intracellular imaging demonstrated that they are potential highly RNA-selective fluorescent turn-on probes possessing good counterstain compatibility with the classic DNA-staining dye DAPI. Moreover, these sensors exhibit large two-photon excited fluorescence action absorption cross-section in the presence of RNA and are also capable of imaging intracellular RNA in two-photon fluorescence microscopy.

Original language	English
Pages (from-to)	329-337
Number of pages	9
Journal	Sensors and Actuators B: Chemical
Volume	173
DOIs	https://doi.org/10.1016/j.snb.2012.07.023
Publication status	Published - Oct 2012

User-Defined Keywords

Bioimaging
Biosensors
Counterstain compatibility
RNA
Two-photon fluorescence

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10.1016/j.snb.2012.07.023

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title = "Fluorescence turn-on probes for intracellular RNA distribution and their imaging in confocal and two-photon fluorescence microscopy",

abstract = "We have synthesized four indole derivatives (INS1, IND1, INS2 and IND2) successfully, and experimental results of biochemical RNA fluorescence assays and direct intracellular imaging demonstrated that they are potential highly RNA-selective fluorescent turn-on probes possessing good counterstain compatibility with the classic DNA-staining dye DAPI. Moreover, these sensors exhibit large two-photon excited fluorescence action absorption cross-section in the presence of RNA and are also capable of imaging intracellular RNA in two-photon fluorescence microscopy.",

keywords = "Bioimaging, Biosensors, Counterstain compatibility, RNA, Two-photon fluorescence",

author = "Guofen Song and Fang Miao and Yuming Sun and Xiaoqiang Yu and Sun, \{Jing Zhi\} and Wong, \{Wai Yeung\}",

note = "Funding Information: For financial support, we thank the National Science Foundation of China ( 50673053 , 50173015 , 30771091 , 50990061 and 50721002 ), NSFC/RGC ( 50218001 ), sklssm201207 , 2011JC006 . W.-Y.W. also acknowledges financial support from the Hong Kong Research Grants Council of HKSAR ( HKBU202709 and HKUST2/CRF/10 ), Areas of Excellence Scheme, University Grants Committee of HKSAR (AoE/P-03/08) and Hong Kong Baptist University (FRG2/10-11/101). ",

year = "2012",

month = oct,

doi = "10.1016/j.snb.2012.07.023",

language = "English",

volume = "173",

pages = "329--337",

journal = "Sensors and Actuators B: Chemical",

issn = "0925-4005",

publisher = "Elsevier B.V.",

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TY - JOUR

T1 - Fluorescence turn-on probes for intracellular RNA distribution and their imaging in confocal and two-photon fluorescence microscopy

AU - Song, Guofen

AU - Miao, Fang

AU - Sun, Yuming

AU - Yu, Xiaoqiang

AU - Sun, Jing Zhi

AU - Wong, Wai Yeung

N1 - Funding Information: For financial support, we thank the National Science Foundation of China ( 50673053 , 50173015 , 30771091 , 50990061 and 50721002 ), NSFC/RGC ( 50218001 ), sklssm201207 , 2011JC006 . W.-Y.W. also acknowledges financial support from the Hong Kong Research Grants Council of HKSAR ( HKBU202709 and HKUST2/CRF/10 ), Areas of Excellence Scheme, University Grants Committee of HKSAR (AoE/P-03/08) and Hong Kong Baptist University (FRG2/10-11/101).

PY - 2012/10

Y1 - 2012/10

N2 - We have synthesized four indole derivatives (INS1, IND1, INS2 and IND2) successfully, and experimental results of biochemical RNA fluorescence assays and direct intracellular imaging demonstrated that they are potential highly RNA-selective fluorescent turn-on probes possessing good counterstain compatibility with the classic DNA-staining dye DAPI. Moreover, these sensors exhibit large two-photon excited fluorescence action absorption cross-section in the presence of RNA and are also capable of imaging intracellular RNA in two-photon fluorescence microscopy.

AB - We have synthesized four indole derivatives (INS1, IND1, INS2 and IND2) successfully, and experimental results of biochemical RNA fluorescence assays and direct intracellular imaging demonstrated that they are potential highly RNA-selective fluorescent turn-on probes possessing good counterstain compatibility with the classic DNA-staining dye DAPI. Moreover, these sensors exhibit large two-photon excited fluorescence action absorption cross-section in the presence of RNA and are also capable of imaging intracellular RNA in two-photon fluorescence microscopy.

KW - Bioimaging

KW - Biosensors

KW - Counterstain compatibility

KW - RNA

KW - Two-photon fluorescence

UR - https://www.scopus.com/pages/publications/84867078847

U2 - 10.1016/j.snb.2012.07.023

DO - 10.1016/j.snb.2012.07.023

M3 - Journal article

AN - SCOPUS:84867078847

SN - 0925-4005

VL - 173

SP - 329

EP - 337

JO - Sensors and Actuators B: Chemical

JF - Sensors and Actuators B: Chemical

ER -

Fluorescence turn-on probes for intracellular RNA distribution and their imaging in confocal and two-photon fluorescence microscopy

Abstract

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