Fluorescence turn-on probes for intracellular RNA distribution and their imaging in confocal and two-photon fluorescence microscopy

Guofen Song, Fang Miao, Yuming Sun, Xiaoqiang Yu*, Jing Zhi Sun, Wai Yeung WONG

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

We have synthesized four indole derivatives (INS1, IND1, INS2 and IND2) successfully, and experimental results of biochemical RNA fluorescence assays and direct intracellular imaging demonstrated that they are potential highly RNA-selective fluorescent turn-on probes possessing good counterstain compatibility with the classic DNA-staining dye DAPI. Moreover, these sensors exhibit large two-photon excited fluorescence action absorption cross-section in the presence of RNA and are also capable of imaging intracellular RNA in two-photon fluorescence microscopy.

Original languageEnglish
Pages (from-to)329-337
Number of pages9
JournalSensors and Actuators, B: Chemical
Volume173
DOIs
Publication statusPublished - Oct 2012

Scopus Subject Areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

User-Defined Keywords

  • Bioimaging
  • Biosensors
  • Counterstain compatibility
  • RNA
  • Two-photon fluorescence

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