Fluorescence quenching method for the determination of catechol with gold nanoparticles and tyrosinase hybrid system

Wen Juan Dong; Jin Ping Song; Chuan Dong; Martin M.F. Choi

doi:10.1016/j.cclet.2009.11.043

Fluorescence quenching method for the determination of catechol with gold nanoparticles and tyrosinase hybrid system

Wen Juan Dong, Jin Ping Song, Chuan Dong^*, Martin M.F. Choi

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

22 Citations (Scopus)

Abstract

The determination method of catechol by fluorescence quenching was developed. The assay was based on the combination of the unique property of gold nanoparticles with tyrosinase enzymatic reaction. In the presence of tyrosinase, the fluorescence of gold nanoparticles was quenched by catechol which can be employed to detect catechol. Under the optimal conditions, a linear range 5.0 × 10^-7-1.0 × 10^-3 mol L^-1 and a detection limit 1.0 × 10^-7 mol L^-1 of catechol were obtained. o-Quinone intermediate produced from the enzymatic catalyzed oxidation of catechol was considered to play the main role in the fluorescence quenching.

Original language	English
Pages (from-to)	346-348
Number of pages	3
Journal	Chinese Chemical Letters
Volume	21
Issue number	3
DOIs	https://doi.org/10.1016/j.cclet.2009.11.043
Publication status	Published - Mar 2010

Scopus Subject Areas

Chemistry(all)

User-Defined Keywords

Catechol
Fluorescence quenching
Gold nanoparticles
Tyrosinase

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10.1016/j.cclet.2009.11.043

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title = "Fluorescence quenching method for the determination of catechol with gold nanoparticles and tyrosinase hybrid system",

abstract = "The determination method of catechol by fluorescence quenching was developed. The assay was based on the combination of the unique property of gold nanoparticles with tyrosinase enzymatic reaction. In the presence of tyrosinase, the fluorescence of gold nanoparticles was quenched by catechol which can be employed to detect catechol. Under the optimal conditions, a linear range 5.0 × 10-7-1.0 × 10-3 mol L-1 and a detection limit 1.0 × 10-7 mol L-1 of catechol were obtained. o-Quinone intermediate produced from the enzymatic catalyzed oxidation of catechol was considered to play the main role in the fluorescence quenching.",

keywords = "Catechol, Fluorescence quenching, Gold nanoparticles, Tyrosinase",

author = "Dong, {Wen Juan} and Song, {Jin Ping} and Chuan Dong and Choi, {Martin M.F.}",

note = "Funding Information: This work was supported by the National Natural Science Foundation of China (No. 20875059 ).",

year = "2010",

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doi = "10.1016/j.cclet.2009.11.043",

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T1 - Fluorescence quenching method for the determination of catechol with gold nanoparticles and tyrosinase hybrid system

AU - Dong, Wen Juan

AU - Song, Jin Ping

AU - Dong, Chuan

AU - Choi, Martin M.F.

N1 - Funding Information: This work was supported by the National Natural Science Foundation of China (No. 20875059 ).

PY - 2010/3

Y1 - 2010/3

N2 - The determination method of catechol by fluorescence quenching was developed. The assay was based on the combination of the unique property of gold nanoparticles with tyrosinase enzymatic reaction. In the presence of tyrosinase, the fluorescence of gold nanoparticles was quenched by catechol which can be employed to detect catechol. Under the optimal conditions, a linear range 5.0 × 10-7-1.0 × 10-3 mol L-1 and a detection limit 1.0 × 10-7 mol L-1 of catechol were obtained. o-Quinone intermediate produced from the enzymatic catalyzed oxidation of catechol was considered to play the main role in the fluorescence quenching.

AB - The determination method of catechol by fluorescence quenching was developed. The assay was based on the combination of the unique property of gold nanoparticles with tyrosinase enzymatic reaction. In the presence of tyrosinase, the fluorescence of gold nanoparticles was quenched by catechol which can be employed to detect catechol. Under the optimal conditions, a linear range 5.0 × 10-7-1.0 × 10-3 mol L-1 and a detection limit 1.0 × 10-7 mol L-1 of catechol were obtained. o-Quinone intermediate produced from the enzymatic catalyzed oxidation of catechol was considered to play the main role in the fluorescence quenching.

KW - Catechol

KW - Fluorescence quenching

KW - Gold nanoparticles

KW - Tyrosinase

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DO - 10.1016/j.cclet.2009.11.043

M3 - Journal article

AN - SCOPUS:74649086638

SN - 1001-8417

VL - 21

SP - 346

EP - 348

JO - Chinese Chemical Letters

JF - Chinese Chemical Letters

IS - 3

ER -

Fluorescence quenching method for the determination of catechol with gold nanoparticles and tyrosinase hybrid system

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