TY - JOUR
T1 - Fluorescence quenching for chloramphenicol detection in milk based on protein-stabilized Au nanoclusters
AU - Tan, Zhijing
AU - Xu, Hua
AU - Li, Gu
AU - Yang, Xiupei
AU - Choi, Martin M.F.
N1 - Funding Information:
This work is supported by the National Natural Science Foundation of China ( 21277109 ) and the Program for Young Scientific and Technological Innovative Research Team in Sichuan Province ( 2014TD0020 ).
PY - 2015/10/5
Y1 - 2015/10/5
N2 - In the present study, we report a simple and rapid method for sensitive and selective determination of chloramphenicol (CAP) based on fluorescence of bovine serum albumin-stabilized Au nanoclusters (BSA-AuNCs). The BSA-AuNCs exhibit strong red emission. Upon addition of CAP to BSA-AuNCs, the fluorescence intensity of AuNCs shows a dramatic decrease attributing to the photo-induced electron transfer process from the electrostatically attached CAP to the BSA-AuNCs. The effects of pH, amount of BSA-AuNCs, temperature and reaction time on the detection of chloramphenicol were investigated. Under the optimal conditions, trace amounts of CAP could be detected. The linear working range is 0.10-70.00 μM with a detection limit 33 nM (S/N = 3). In addition, the proposed method has been successfully applied to the detection of CAP in milk samples and largely improves the application of spectral method for quantitative analysis of CAP.
AB - In the present study, we report a simple and rapid method for sensitive and selective determination of chloramphenicol (CAP) based on fluorescence of bovine serum albumin-stabilized Au nanoclusters (BSA-AuNCs). The BSA-AuNCs exhibit strong red emission. Upon addition of CAP to BSA-AuNCs, the fluorescence intensity of AuNCs shows a dramatic decrease attributing to the photo-induced electron transfer process from the electrostatically attached CAP to the BSA-AuNCs. The effects of pH, amount of BSA-AuNCs, temperature and reaction time on the detection of chloramphenicol were investigated. Under the optimal conditions, trace amounts of CAP could be detected. The linear working range is 0.10-70.00 μM with a detection limit 33 nM (S/N = 3). In addition, the proposed method has been successfully applied to the detection of CAP in milk samples and largely improves the application of spectral method for quantitative analysis of CAP.
KW - Bovine serum albumin
KW - Chloramphenicol
KW - Fluorescence
KW - Gold nanoclusters
UR - http://www.scopus.com/inward/record.url?scp=84929300095&partnerID=8YFLogxK
U2 - 10.1016/j.saa.2015.04.109
DO - 10.1016/j.saa.2015.04.109
M3 - Journal article
C2 - 25985125
AN - SCOPUS:84929300095
SN - 1386-1425
VL - 149
SP - 615
EP - 620
JO - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
JF - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
ER -