Flavonoids are indispensable for complete male fertility in rice

Lanxiang Wang, Pui Ying Lam, Andy C.W. Lui, Fu Yuan Zhu, Mo Xian Chen, Hongjia Liu, Jianhua ZHANG, Clive Lo*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Flavonoids are essential for male fertility in some but not all plant species. In rice (Oryza sativa), the chalcone synthase mutant oschs1 produces flavonoid-depleted pollen and is male sterile. The mutant pollen grains are viable with normal structure, but they display reduced germination rate and pollen-tube length. Analysis of oschs1/+ heterozygous lines shows that pollen flavonoid deposition is a paternal effect and fertility is independent of the haploid genotypes (OsCHS1 or oschs1). To understand which classes of flavonoids are involved in male fertility, we conducted detailed analysis of rice mutants for branch-point enzymes of the downstream flavonoid pathways, including flavanone 3-hydroxylase (OsF3H; flavonol pathway entry enzyme), flavone synthase II (CYP93G1; flavone pathway entry enzyme), and flavanone 2-hydroxylase (CYP93G2; flavone C-glycoside pathway entry enzyme). Rice osf3h and cyp93g1 cyp93g2 CRISPR/Cas9 mutants, and cyp93g1 and cyp93g2 T-DNA insertion mutants showed altered flavonoid profiles in anthers, but only the osf3h and cyp93g1 cyp93g2 mutants displayed reduction in seed yield. Our findings indicate that flavonoids are essential for complete male fertility in rice and a combination of different classes (flavanones, flavonols, flavones, and flavone C-glycosides) appears to be important, as opposed to the essential role played primarily by flavonols that has been previously reported in several plant species.

Original languageEnglish
Pages (from-to)4715-4728
Number of pages14
JournalJournal of Experimental Botany
Volume71
Issue number16
DOIs
Publication statusPublished - 1 Aug 2020

Scopus Subject Areas

  • Physiology
  • Plant Science

User-Defined Keywords

  • Chalcone synthase
  • Flavanones
  • Flavone C-glycosides
  • Flavones
  • Flavonols
  • Male fertility
  • Oryza sativa
  • Rice

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