Fine particulate matter induces endoplasmic reticulum stress-mediated apoptosis in human SH-SY5Y cells

Mei Zhang, Ying Wang , Ricky M.S. Wong, Ken Kin Lam Yung*, Ruijin Li*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Exposure to ambient fine particulate matter (PM2.5) may contribute to brain injury, however, the molecular mechanisms have not yet been fully described. In this study, the human SH-SY5Y cells were treated with PM2.5 with different concentrations (0, 25, 100, and 250 μg/mL) for 24 h to investigate the cell apoptosis mediated by endoplasmic reticulum (ER) stress. The ratio of apoptosis, Ca2+ level, biomarkers of ER stress and apoptosis were determined. The results revealed that PM2.5 triggered the increase of apoptosis ratio and cellular Ca2+ levels. Compared with control, the expression of GRP78 and phosphorylation of IER1α and p38 were enhanced significantly in the cells under the conditions of PM2.5 exposure for activating ER stress signals. Besides, the key genes (CHOP/DR5/Caspase8/Caspase12) in ER stress-induced apoptosis signals were up-regulated after the PM2.5 treatment compared to the control. The results suggested PM2.5 induced apoptosis in SH-SY5Y cells by the stimulation of ER stress, which may be the potential mechanism of neurological diseases incurred by PM2.5.

Original languageEnglish
Pages (from-to)187-195
Number of pages9
JournalNeuroToxicology
Volume88
Early online date20 Nov 2021
DOIs
Publication statusPublished - Jan 2022

Scopus Subject Areas

  • Neuroscience(all)
  • Toxicology

User-Defined Keywords

  • Apoptosis
  • Endoplasmic reticulum stress
  • Fine particulate matter
  • Human SH-SY5Y cells

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