Ficus virens proanthocyanidins induced apoptosis in breast cancer cells concomitantly ameliorated 5-fluorouracil induced intestinal mucositis in rats

Xiaoxin Chen, Kar Ho Lam, Qingxi Chen, George Pak Heng Leung, Sydney Chi Wai Tang, Stephen Cho Wing SZE, Jian-Bo Xiao, Feng Feng, Ying Wang, Kalin Yanbo Zhang*, Zhangjin Zhang *

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

5-Fluorouracil (5-FU) is a commonly used chemotherapeutic agent for breast cancer. However, its use often leads to drug resistance and mucositis. This study aimed to investigate whether proanthocyanidins from Ficus virens possessed anti-breast cancer and anti-mucositis activities. The results showed that the cytotoxic effects of the proanthocyanidins against MDA-MB-231 and MCF-7 breast cancer cells were in the order of stem barks proanthocyanidins (SPAs) > leaves proanthocyanidins > fruits proanthocyanidins. Moreover, SPAs induced apoptosis in both cell lines which were accompanied with an increase in loss of mitochondrial membrane potential, production of reactive oxygen species, Bax to Bcl-2 protein expression ratio, and activated caspase 3. Furthermore, intraperitoneal injection of 5-FU (150 mg/kg body weight) resulted in body weight loss and jejunal injury in the rats while administration of SPAs (100 mg/kg body weight) counteracted these changes. Collectively, our study demonstrated that SPAs induced apoptosis cell death in breast cancer cells while ameliorating the symptoms of intestinal mucositis in rats.Therefore, SPAs merits further exploration as a potential therapeutic agent for breast cancer and chemotherapy-induced mucositis.
Original languageEnglish
Pages (from-to)49-61
Number of pages13
JournalFood and Chemical Toxicology
Volume110
DOIs
Publication statusPublished - Dec 2017

Scopus Subject Areas

  • Medicine(all)

User-Defined Keywords

  • Ficus virens
  • Proanthocyanidins
  • Breast cancer
  • Apoptosis
  • 5-Fluorouracil
  • Mucositis

Fingerprint

Dive into the research topics of 'Ficus virens proanthocyanidins induced apoptosis in breast cancer cells concomitantly ameliorated 5-fluorouracil induced intestinal mucositis in rats'. Together they form a unique fingerprint.

Cite this