TY - JOUR
T1 - Ficus virens proanthocyanidins induced apoptosis in breast cancer cells concomitantly ameliorated 5-fluorouracil induced intestinal mucositis in rats
AU - Chen, Xiaoxin
AU - Lam, Kar Ho
AU - Chen, Qingxi
AU - Leung, George Pak Heng
AU - Tang, Sydney Chi Wai
AU - SZE, Stephen Cho Wing
AU - Xiao, Jian-Bo
AU - Feng, Feng
AU - Wang, Ying
AU - Zhang, Kalin Yanbo
AU - Zhang , Zhangjin
PY - 2017/12
Y1 - 2017/12
N2 - 5-Fluorouracil (5-FU) is a commonly used chemotherapeutic agent for breast cancer. However, its use often leads to drug resistance and mucositis. This study aimed to investigate whether proanthocyanidins from Ficus virens possessed anti-breast cancer and anti-mucositis activities. The results showed that the cytotoxic effects of the proanthocyanidins against MDA-MB-231 and MCF-7 breast cancer cells were in the order of stem barks proanthocyanidins (SPAs) > leaves proanthocyanidins > fruits proanthocyanidins. Moreover, SPAs induced apoptosis in both cell lines which were accompanied with an increase in loss of mitochondrial membrane potential, production of reactive oxygen species, Bax to Bcl-2 protein expression ratio, and activated caspase 3. Furthermore, intraperitoneal injection of 5-FU (150 mg/kg body weight) resulted in body weight loss and jejunal injury in the rats while administration of SPAs (100 mg/kg body weight) counteracted these changes. Collectively, our study demonstrated that SPAs induced apoptosis cell death in breast cancer cells while ameliorating the symptoms of intestinal mucositis in rats.Therefore, SPAs merits further exploration as a potential therapeutic agent for breast cancer and chemotherapy-induced mucositis.
AB - 5-Fluorouracil (5-FU) is a commonly used chemotherapeutic agent for breast cancer. However, its use often leads to drug resistance and mucositis. This study aimed to investigate whether proanthocyanidins from Ficus virens possessed anti-breast cancer and anti-mucositis activities. The results showed that the cytotoxic effects of the proanthocyanidins against MDA-MB-231 and MCF-7 breast cancer cells were in the order of stem barks proanthocyanidins (SPAs) > leaves proanthocyanidins > fruits proanthocyanidins. Moreover, SPAs induced apoptosis in both cell lines which were accompanied with an increase in loss of mitochondrial membrane potential, production of reactive oxygen species, Bax to Bcl-2 protein expression ratio, and activated caspase 3. Furthermore, intraperitoneal injection of 5-FU (150 mg/kg body weight) resulted in body weight loss and jejunal injury in the rats while administration of SPAs (100 mg/kg body weight) counteracted these changes. Collectively, our study demonstrated that SPAs induced apoptosis cell death in breast cancer cells while ameliorating the symptoms of intestinal mucositis in rats.Therefore, SPAs merits further exploration as a potential therapeutic agent for breast cancer and chemotherapy-induced mucositis.
KW - Ficus virens
KW - Proanthocyanidins
KW - Breast cancer
KW - Apoptosis
KW - 5-Fluorouracil
KW - Mucositis
UR - http://www.scopus.com/inward/record.url?eid=2-s2.0-85031124022&partnerID=MN8TOARS
U2 - 10.1016/j.fct.2017.10.017
DO - 10.1016/j.fct.2017.10.017
M3 - Journal article
SN - 0278-6915
VL - 110
SP - 49
EP - 61
JO - Food and Chemical Toxicology
JF - Food and Chemical Toxicology
ER -