Feasibility of Sijunzi Tang (Chinese medicine) to enhance protein disulfide isomerase activities for reactivating malate dehydrogenase deactivated by polycyclic aromatic hydrocarbons

Ho Man Leung*, Francis Siu Lai Kwok, Wing Yin Mo, Kwai Chung Cheung, Yik Kit Yue, Yee Keung Wong, Chi Kin Au, Martin Tsz Ki Tsui, Kin Lam YUNG

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The objective of this research is to investigate the enzymatic activities between protein disulfide isomerase (PDI) found in animals and plants and the properties found in a commonly used Chinese medicine called Sijunzi Tang. During the investigation, PDI, which is a monomer with a molecular mass of 57.0 kDa, was used to reactivate malate dehydrogenase (MDH). However, with the interference of polycyclic aromatic hydrocarbons (PAHs), evidence indicates that such chemicals are carcinogenic, mutagenic, and toxic to humans. The enzymatic activity of PDI found in animal’s liver and plant was 1657 folds of purification; 0.284 unit/mg of enzyme activity, and 5694.4 folds of purification; 1.00 unit/mg of enzyme activity, respectively. PDI extracted in treated animal and plant tissue revealed 2.40% and 80.44% of regaining MDH enzymatic activity, respectively. Although in its initial phase of investigation, it is assumed that the properties found in Sijunzi Tang can help regain enzymatic activity in those affected by xenobiotic substances, thus, making it a potential ingredient in assisting with PDI functions.

Original languageEnglish
JournalEnvironmental Science and Pollution Research
DOIs
Publication statusAccepted/In press - 2018

Scopus Subject Areas

  • Environmental Chemistry
  • Pollution
  • Health, Toxicology and Mutagenesis

User-Defined Keywords

  • Glutathione reductase assay
  • Malate dehydrogenase (MDH)
  • Polycyclic aromatic hydrocarbons (PAHs)
  • Protein disulfide isomerase (PDI)
  • Sijunzi Tang

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