TY - JOUR
T1 - Facile synthesis of spherical covalent organic frameworks for enrichment and quantification of aryl organophosphate esters in mouse serum and tissues
AU - Wu, Yijing
AU - Zheng, Wenjun
AU - Chen, Canrong
AU - Yang, Linyan
AU - Tong, Ping
AU - Zhong, Yanhui
AU - Lin, Zian
AU - Cai, Zongwei
N1 - This work was supported by the National Natural Science Foundation of China (22036001, 91843301, and 21974021), the Natural Science Foundation of Fujian Province (2022J01535), and the Major Project of Science and Technology of Fujian Province (2020HZ06006).
Publisher Copyright:
© 2023 Wiley-VCH GmbH.
PY - 2023/11
Y1 - 2023/11
N2 - Here, an imine-linked-based spherical covalent organic framework (COF) was prepared at room temperature. The as-synthesized spherical COF served as an adsorbent in dispersive solid-phase extraction (dSPE), by its virtue of great surface area (1542.68 m2/g), regular distribution of pore size (2.95 nm), and excellent stability. Therefore, a simple and high-efficiency dispersive solid phase extraction method based on a spherical COF coupled with high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was established to determine aryl organophosphate esters in biological samples. This approach displayed favorable linearity in the range of 10.0–1000.0 ng/L (r > 0.9989), a high signal enhancement factor (58.8–181.8 folds) with low limits of detection (0.3–3.3 ng/L). Moreover, it could effectively eliminate complex matrix interference to accurately extract seven aryl organophosphate esters from mouse serum and tissue samples with spiked recoveries of 82.0%–117.4%. The as-synthesized spherical COF has been successfully applied in sample preparation. The dSPE-HPLC-MS/MS method based on a spherical COF has potential application to study the pollutants' metabolism in vivo.
AB - Here, an imine-linked-based spherical covalent organic framework (COF) was prepared at room temperature. The as-synthesized spherical COF served as an adsorbent in dispersive solid-phase extraction (dSPE), by its virtue of great surface area (1542.68 m2/g), regular distribution of pore size (2.95 nm), and excellent stability. Therefore, a simple and high-efficiency dispersive solid phase extraction method based on a spherical COF coupled with high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was established to determine aryl organophosphate esters in biological samples. This approach displayed favorable linearity in the range of 10.0–1000.0 ng/L (r > 0.9989), a high signal enhancement factor (58.8–181.8 folds) with low limits of detection (0.3–3.3 ng/L). Moreover, it could effectively eliminate complex matrix interference to accurately extract seven aryl organophosphate esters from mouse serum and tissue samples with spiked recoveries of 82.0%–117.4%. The as-synthesized spherical COF has been successfully applied in sample preparation. The dSPE-HPLC-MS/MS method based on a spherical COF has potential application to study the pollutants' metabolism in vivo.
KW - aryl organophosphate esters
KW - biological samples
KW - covalent organic frameworks
KW - dispersive solid phase extraction
KW - quantification
UR - http://www.scopus.com/inward/record.url?scp=85171470411&partnerID=8YFLogxK
U2 - 10.1002/jssc.202300482
DO - 10.1002/jssc.202300482
M3 - Journal article
C2 - 37727055
AN - SCOPUS:85171470411
SN - 1615-9306
VL - 46
JO - Journal of Separation Science
JF - Journal of Separation Science
IS - 22
M1 - 2300482
ER -