Fabrication of a microfluidic Ag/AgCl reference electrode and its application for portable and disposable electrochemical microchips

Jianhua Zhou, Kangning REN, Yizhe Zheng, Jing Su, Yihua Zhao, Declan Ryan, Hongkai Wu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

48 Citations (Scopus)

Abstract

This report describes a convenient method for the fabrication of a miniaturized, reliable Ag/AgCl reference electrode with nanofluidic channels acting as a salt bridge that can be easily integrated into microfluidic chips. The Ag/AgCl reference electrode shows high stability with millivolt variations. We demonstrated the application of this reference electrode in a portable microfluidic chip that is connected to a USB-port microelectrochemical station and to a computer for data collection and analysis. The low fabrication cost of the chip with the potential for mass production makes it disposable and an excellent candidate for real-world analysis and measurement. We used the chip to quantitatively analyze the concentrations of heavy metal ions (Cd2+ and Pb2+) in sea water. We believe that the Ag/AgCl reference microelectrode and the portable electrochemical system will be of interest to people in microfluidics, environmental science, clinical diagnostics, and food research.

Original languageEnglish
Pages (from-to)3083-3089
Number of pages7
JournalElectrophoresis
Volume31
Issue number18
DOIs
Publication statusPublished - Sep 2010

Scopus Subject Areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

User-Defined Keywords

  • Electrochemical chip
  • Heavy metal ion detection
  • Integrated Ag/AgCl electrode
  • Microfluidics
  • Portable device

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