Abstract
Objective: To observe the effects of water extract of Zuojin Pill (左金丸, ZJP) on inhibiting the growth of human gastric cancer cell line SGC-7901 and its potential mechanism. Methods: Effects of ZJP on SGC-7901 cells growth were determined by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay, cell apoptosis and cell cycle were determined by flow cytometry, and apoptosis induction was detected by means of DNA gel electrophoresis. The cellular mechanism of drug-induced cell death was unraveled by assaying oxidative injury level of SGC-7901 cell, mitochondrial membrane potentials, expression of apoptosis-related genes, such as B cell lymphoma/lewkmia-2 (Bcl-2), Bcl-2 associated X protein (Bax) and cleaved caspase-3 and caspase-9. Results: ZJP exerted evident inhibitory effect on SGC-7901 cells by activating production of reactive oxygen species and elevating Bax/Bcl-2 ratio in SGC-7901 cells, leading to attenuation of mitochondrial membrane potential and DNA fragmentation. Conclusions: ZJP inhibits the cancer cell growth via activating mitochondria-dependent apoptosis pathway. ZJP can potentially serve as an antitumor agent.
Original language | English |
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Pages (from-to) | 837-845 |
Number of pages | 9 |
Journal | Chinese Journal of Integrative Medicine |
Volume | 21 |
Issue number | 11 |
Early online date | 6 Apr 2015 |
DOIs | |
Publication status | Published - Nov 2015 |
Scopus Subject Areas
- Complementary and alternative medicine
- Pharmacology (medical)
User-Defined Keywords
- apoptosis
- Chinese medicine
- mitochondria-dependent pathway
- reactive oxygen species
- Zuojin Pill