Extract of Zuojin Pill (左金丸) induces apoptosis of SGC-7901 cells via mitochondria-dependent pathway

Qiu xian Peng, Hong bing Cai, Jiang li Peng, Kin Lam YUNG, Jue SHI, Zhi xian Mo*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Objective: To observe the effects of water extract of Zuojin Pill (左金丸, ZJP) on inhibiting the growth of human gastric cancer cell line SGC-7901 and its potential mechanism. Methods: Effects of ZJP on SGC-7901 cells growth were determined by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay, cell apoptosis and cell cycle were determined by flow cytometry, and apoptosis induction was detected by means of DNA gel electrophoresis. The cellular mechanism of drug-induced cell death was unraveled by assaying oxidative injury level of SGC-7901 cell, mitochondrial membrane potentials, expression of apoptosis-related genes, such as B cell lymphoma/lewkmia-2 (Bcl-2), Bcl-2 associated X protein (Bax) and cleaved caspase-3 and caspase-9. Results: ZJP exerted evident inhibitory effect on SGC-7901 cells by activating production of reactive oxygen species and elevating Bax/Bcl-2 ratio in SGC-7901 cells, leading to attenuation of mitochondrial membrane potential and DNA fragmentation. Conclusions: ZJP inhibits the cancer cell growth via activating mitochondria-dependent apoptosis pathway. ZJP can potentially serve as an antitumor agent.

Original languageEnglish
Pages (from-to)837-845
Number of pages9
JournalChinese Journal of Integrative Medicine
Volume21
Issue number11
DOIs
Publication statusPublished - 6 Apr 2015

Scopus Subject Areas

  • Complementary and alternative medicine
  • Pharmacology (medical)

User-Defined Keywords

  • apoptosis
  • Chinese medicine
  • mitochondria-dependent pathway
  • reactive oxygen species
  • Zuojin Pill

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