Expression of TRPC homologs in endothelial cells and smooth muscle layers of human arteries

Ham Yip, Wing Yee Chan, Pan Cheung Leung, Hiu Yee Kwan, Culling Liu, Yu Huang, Villaz Michel, David Tai Wai Yew, Xiaoqiang Yao*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

89 Citations (Scopus)


TRPC channels are a group of Ca2+-permeable nonselective cation channels that mediate store-operated and/or agonist-stimulated Ca2+ influx in a variety of cell types. In this study, we extensively examined the expression patterns of TRPC homologs in human vascular tissues. RT-PCR amplified cDNA fragments of TRPC1 (505 bp), TRPC3 (372 bp), TRPC4 (499 bp), TRPC5 (325 bp), TRPC6 (509 bp), and TRPC7 (187 bp) from RNA isolated from cultured human coronary artery endothelial cells. In situ hybridization yielded strong labeling of TRPC 1,3-6 in the endothelial and smooth muscle cells of human coronary and cerebral arteries. TRPC7 labeling was exclusively found in endothelial cells but not in smooth muscle cells. Results from immunohistochemical staining were consistent with those from in situ hybridization. Similar expression patterns of TRPC homologs were also observed in arterioles and vaso vasora. In conclusion, our study indicates that TRPC homologs are widely expressed in human vessels of all calibers, including medium-sized coronary arteries and cerebral arteries, smaller-sized resistance arteries, and vaso vasora. These results suggest a ubiquitous role of TRPC homologs in regulating blood supply to different regions and in controlling arterial blood pressure.

Original languageEnglish
Pages (from-to)553-561
Number of pages9
JournalHistochemistry and Cell Biology
Issue number6
Early online date5 Nov 2004
Publication statusPublished - Dec 2004

Scopus Subject Areas

  • Histology
  • Molecular Biology
  • Medical Laboratory Technology
  • Cell Biology

User-Defined Keywords

  • Ca2+ channel
  • Endothelium
  • Gene expression
  • Immunohistochemistry
  • Smooth muscle


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