Expression of an Avian Influenza Virus (H5N1) Hemagglutinin Gene in Transgenic Lotus corniculatus

Qian Qian Guo; Zhan Lu Zhang; San Jie Jiang; Jiang Tao Ma; Wen Tong Xue; Yan Min Wu

doi:10.1007/s11105-012-0423-9

Expression of an Avian Influenza Virus (H5N1) Hemagglutinin Gene in Transgenic Lotus corniculatus

Qian Qian Guo
, Zhan Lu Zhang
, San Jie Jiang
, Jiang Tao Ma
, Wen Tong Xue^*
, Yan Min Wu^*

^*Corresponding author for this work

Department of Biology

Research output: Contribution to journal › Journal article › peer-review

8 Citations (Scopus)

Abstract

Cotyledons from 8-day-old seedlings of Lotus corniculatus were transformed with a gene encoding the avian influenza (H5N1) hemagglutinin (HA) under the control of the CaMV35S promoter along with matrix attachment regions using Agrobacterium-mediated transformation. The HA gene was detected in genomic DNA from transformed plants by PCR and Southern blotting. Immunoblot analysis of the plant-derived protein revealed the presence of the avian influenza (H5N1) HA. The ELISA results showed that the maximum expression level of the HA protein is up to 0.00786% of the total soluble protein. This is the first report of successful expression of the avian influenza (H5N1) HA in transgenic L. corniculatus, cultivar Leo.

Original language	English
Pages (from-to)	1117-1124
Number of pages	8
Journal	Plant Molecular Biology Reporter
Volume	30
Issue number	5
DOIs	https://doi.org/10.1007/s11105-012-0423-9
Publication status	Published - Oct 2012

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This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

User-Defined Keywords

H5N1
Hemagglutinin
Lotus corniculatus
Matrix attachment regions
Plant bioreactor

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10.1007/s11105-012-0423-9

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@article{79ad15c498614d2ead949e6db939dba5,

title = "Expression of an Avian Influenza Virus (H5N1) Hemagglutinin Gene in Transgenic Lotus corniculatus",

abstract = "Cotyledons from 8-day-old seedlings of Lotus corniculatus were transformed with a gene encoding the avian influenza (H5N1) hemagglutinin (HA) under the control of the CaMV35S promoter along with matrix attachment regions using Agrobacterium-mediated transformation. The HA gene was detected in genomic DNA from transformed plants by PCR and Southern blotting. Immunoblot analysis of the plant-derived protein revealed the presence of the avian influenza (H5N1) HA. The ELISA results showed that the maximum expression level of the HA protein is up to 0.00786\% of the total soluble protein. This is the first report of successful expression of the avian influenza (H5N1) HA in transgenic L. corniculatus, cultivar Leo.",

keywords = "H5N1, Hemagglutinin, Lotus corniculatus, Matrix attachment regions, Plant bioreactor",

author = "Guo, \{Qian Qian\} and Zhang, \{Zhan Lu\} and Jiang, \{San Jie\} and Ma, \{Jiang Tao\} and Xue, \{Wen Tong\} and Wu, \{Yan Min\}",

note = "Publisher Copyright: {\textcopyright} Springer-Verlag 2012. Funding Information: We thank Zhao Yang-min for technical assistance in RNA extraction, Tao Fei for isolating genomic DNA, and Yuan Bei for her help in genetic transformation. This work was supported by the Special Funds for Major State Basic Research Program of China (973 Program) (2007CB10890222).",

year = "2012",

month = oct,

doi = "10.1007/s11105-012-0423-9",

language = "English",

volume = "30",

pages = "1117--1124",

journal = "Plant Molecular Biology Reporter",

issn = "0735-9640",

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}

TY - JOUR

T1 - Expression of an Avian Influenza Virus (H5N1) Hemagglutinin Gene in Transgenic Lotus corniculatus

AU - Guo, Qian Qian

AU - Zhang, Zhan Lu

AU - Jiang, San Jie

AU - Ma, Jiang Tao

AU - Xue, Wen Tong

AU - Wu, Yan Min

N1 - Publisher Copyright: © Springer-Verlag 2012. Funding Information: We thank Zhao Yang-min for technical assistance in RNA extraction, Tao Fei for isolating genomic DNA, and Yuan Bei for her help in genetic transformation. This work was supported by the Special Funds for Major State Basic Research Program of China (973 Program) (2007CB10890222).

PY - 2012/10

Y1 - 2012/10

N2 - Cotyledons from 8-day-old seedlings of Lotus corniculatus were transformed with a gene encoding the avian influenza (H5N1) hemagglutinin (HA) under the control of the CaMV35S promoter along with matrix attachment regions using Agrobacterium-mediated transformation. The HA gene was detected in genomic DNA from transformed plants by PCR and Southern blotting. Immunoblot analysis of the plant-derived protein revealed the presence of the avian influenza (H5N1) HA. The ELISA results showed that the maximum expression level of the HA protein is up to 0.00786% of the total soluble protein. This is the first report of successful expression of the avian influenza (H5N1) HA in transgenic L. corniculatus, cultivar Leo.

AB - Cotyledons from 8-day-old seedlings of Lotus corniculatus were transformed with a gene encoding the avian influenza (H5N1) hemagglutinin (HA) under the control of the CaMV35S promoter along with matrix attachment regions using Agrobacterium-mediated transformation. The HA gene was detected in genomic DNA from transformed plants by PCR and Southern blotting. Immunoblot analysis of the plant-derived protein revealed the presence of the avian influenza (H5N1) HA. The ELISA results showed that the maximum expression level of the HA protein is up to 0.00786% of the total soluble protein. This is the first report of successful expression of the avian influenza (H5N1) HA in transgenic L. corniculatus, cultivar Leo.

KW - H5N1

KW - Hemagglutinin

KW - Lotus corniculatus

KW - Matrix attachment regions

KW - Plant bioreactor

UR - https://www.scopus.com/pages/publications/84865432198

U2 - 10.1007/s11105-012-0423-9

DO - 10.1007/s11105-012-0423-9

M3 - Journal article

AN - SCOPUS:84865432198

SN - 0735-9640

VL - 30

SP - 1117

EP - 1124

JO - Plant Molecular Biology Reporter

JF - Plant Molecular Biology Reporter

IS - 5

ER -

Expression of an Avian Influenza Virus (H5N1) Hemagglutinin Gene in Transgenic Lotus corniculatus

Abstract

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