TY - JOUR
T1 - Exposure to ambient fine particulate matter impedes the function of spleen in the mouse metabolism of high-fat diet
AU - He, Zhao
AU - Zhang, Hongna
AU - Song, Yuanyuan
AU - Yang, Zhu
AU - Cai, Zongwei
N1 - Funding Information:
We gratefully acknowledge the fund support of Major Research Plan 91843301 from the National Natural Science Foundation of China (NSFC), National Key R&D Program 2018YFA0901100 from Ministry of Science and Technology , China, internal research funds RC-SGT2/18–19/SCI/008 and SKLP_1920_P07 from Hong Kong Baptist University , Hong Kong, China, General Research Fund (GRF) 12103820 from University Grants Committee (UGC), Hong Kong, China, and SGDX20190816230207535 from Shenzhen Science and Technology Innovation Commission (SZSTC), China. The authors also thank Dr. Lin Zhu and Ms. Xiaoxiao Wang of the State Key Laboratory of Environmental and Biological analysis for the instruction and assists in the application of instruments.
Publisher Copyright:
© 2021 Elsevier B.V.
PY - 2022/2/5
Y1 - 2022/2/5
N2 - Epidemiological and experimental evidence has been associating the exposure with ambient fine particulate matter (PM2.5) with metabolic malfunctions such as obesity and cardiovascular disease. As the blood-filter and the important lymphatic organ, spleen participates in the regulation of metabolic balance. In this work, liquid chromatography–mass spectrometry (LC-MS)-based lipidomics, metabolomics and proteomics were performed to study the effects of PM2.5 exposure and high-fat diet (HFD) induced obesity on mice spleen. By comparing the differences in lipids, metabolites, and proteins in the spleens from PM2.5 and HFD treated mice, we discovered the individual and combined effects of the two risk factors. The results showed the PM2.5 exposure altered energy metabolism of the mice, as evidenced by the upregulation of TCA cycle. In addition, the metabolism of branched-chain amino acids was also significantly changed, which might be related to the preventive function of spleen in lipid metabolism. The PM2.5-induced metabolic changes in spleen could further aggravate the adverse impacts of HFD on mice, resulting in impeded splenic metabolism of lipids. This study revealed the effects of PM2.5 and obesity mice spleen, which might be of great significance to public health.
AB - Epidemiological and experimental evidence has been associating the exposure with ambient fine particulate matter (PM2.5) with metabolic malfunctions such as obesity and cardiovascular disease. As the blood-filter and the important lymphatic organ, spleen participates in the regulation of metabolic balance. In this work, liquid chromatography–mass spectrometry (LC-MS)-based lipidomics, metabolomics and proteomics were performed to study the effects of PM2.5 exposure and high-fat diet (HFD) induced obesity on mice spleen. By comparing the differences in lipids, metabolites, and proteins in the spleens from PM2.5 and HFD treated mice, we discovered the individual and combined effects of the two risk factors. The results showed the PM2.5 exposure altered energy metabolism of the mice, as evidenced by the upregulation of TCA cycle. In addition, the metabolism of branched-chain amino acids was also significantly changed, which might be related to the preventive function of spleen in lipid metabolism. The PM2.5-induced metabolic changes in spleen could further aggravate the adverse impacts of HFD on mice, resulting in impeded splenic metabolism of lipids. This study revealed the effects of PM2.5 and obesity mice spleen, which might be of great significance to public health.
KW - HFD
KW - LC-MS
KW - Lipidomics
KW - Metabolomics
KW - Mouse spleen
KW - PM2.5
KW - Proteomics
UR - http://www.scopus.com/inward/record.url?scp=85114688436&partnerID=8YFLogxK
U2 - 10.1016/j.jhazmat.2021.127129
DO - 10.1016/j.jhazmat.2021.127129
M3 - Journal article
C2 - 34509742
AN - SCOPUS:85114688436
SN - 0304-3894
VL - 423, Part B
JO - Journal of Hazardous Materials
JF - Journal of Hazardous Materials
M1 - 127129
ER -
