Expansion Strategy-Driven Micron-Level Resolution Mass Spectrometry Imaging of Lipids in Mouse Brain Tissue

Yik Ling Winnie Hung, Chengyi Xie, Jianing Wang*, Xin Diao, Ruxin Li, Xiaoxiao Wang, Shulan Qiu, Jiacheng Fang, Zongwei Cai*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

2 Citations (Scopus)

Abstract

A novel method for enhanced resolution, termed expansion mass spectrometry imaging, has been developed for lipid mass spectrometry imaging, utilizing existing commercially available mass spectrometers without necessitating modifications. This approach involves embedding tissue sections in a swellable polyelectrolyte gel, with the target biomolecules indirectly anchored to the gel network. By employing matrix-assisted laser desorption ionization mass spectrometry imaging, the method has realized an enhanced spatial resolution that surpasses the conventional resolution limits of commercial instruments by approximately 4.5 fold. This enhancement permits the detailed visualization of intricate structures within the mouse brain at a subcellular level, with a lateral resolution nearing 1 μm. As a physical technique for achieving resolution beyond standard capabilities, this readily adaptable approach presents a powerful tool for high-definition imaging in biological research.

Original languageEnglish
Pages (from-to)2662-2670
Number of pages9
JournalCCS Chemistry
Volume6
Issue number11
DOIs
Publication statusPublished - Nov 2024

Scopus Subject Areas

  • General Chemistry

User-Defined Keywords

  • expansion
  • high resolution
  • MALDI imaging
  • mass spectrometry imaging
  • molecular imaging
  • spatial lipidomics
  • subcellular imaging

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