@article{4aeb1811951f4fd9a4174ed00484a62e,
title = "Expansion Strategy-Driven Micron-Level Resolution Mass Spectrometry Imaging of Lipids in Mouse Brain Tissue",
abstract = "A novel method for enhanced resolution, termed expansion mass spectrometry imaging, has been developed for lipid mass spectrometry imaging, utilizing existing commercially available mass spectrometers without necessitating modifications. This approach involves embedding tissue sections in a swellable polyelectrolyte gel, with the target biomolecules indirectly anchored to the gel network. By employing matrix-assisted laser desorption ionization mass spectrometry imaging, the method has realized an enhanced spatial resolution that surpasses the conventional resolution limits of commercial instruments by approximately 4.5 fold. This enhancement permits the detailed visualization of intricate structures within the mouse brain at a subcellular level, with a lateral resolution nearing 1 μm. As a physical technique for achieving resolution beyond standard capabilities, this readily adaptable approach presents a powerful tool for high-definition imaging in biological research.",
keywords = "expansion, high resolution, MALDI imaging, mass spectrometry imaging, molecular imaging, spatial lipidomics, subcellular imaging",
author = "Hung, {Yik Ling Winnie} and Chengyi Xie and Jianing Wang and Xin Diao and Ruxin Li and Xiaoxiao Wang and Shulan Qiu and Jiacheng Fang and Zongwei Cai",
note = "Funding Information: This study was supported by the General Research Fund (grant no. 12302122) of the Research Grants Council, Hong Kong Special Administrative Region, China, the Start-up Grant from Hong Kong Baptist University, and the State Key Laboratory of Environmental and Biological Analysis Research Grant (grant no. SKLP_2021_P04). We thank Dr. Jie Liu, Southern University of Science and Technology, for her help in fixation and staining experiments in ExM. We thank Dr. Paul Tillberg, Janelia Research Campus, Howard Hughes Medical Institute, for the consultant and discussion on ExM. We thank Dr. Fei Chen, Broad Institute of Massachusetts Institute of Technology and Harvard, for the consultant on ExM experiments. We thank Dr. Lin Zhu and Dr. Zhu Yang, Hong Kong Baptist University, for the discussion of relevant academic issues. Publisher Copyright: {\textcopyright} 2024 Chinese Chemical Society. All rights reserved.",
year = "2024",
month = nov,
doi = "10.31635/ccschem.024.202404002",
language = "English",
volume = "6",
pages = "2662--2670",
journal = "CCS Chemistry",
issn = "2096-5745",
publisher = "Chinese Chemical Society",
number = "11",
}
