Exogenous phytoestrogenic molecule icaritin incorporated into a porous scaffold for enhancing bone defect repair

Xin Luan Wang*, Xin Hui Xie, Ge ZHANG, Shi Hui Chen, Dong Yao, Kai He, Xiao Hong Wang, Xin Sheng Yao, Yang Leng, Kwok Pui Fung, Kwok Sui Leung, Ling Qin

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

40 Citations (Scopus)

Abstract

This study was designed to develop a bioactive scaffold to enhance bone defect repair in steroid-associated osteonecrosis (SAON). Icaritin, a metabolite of the herb Epimedium, has been identified as an angiogenic and osteogenic phytomolecule. Icaritin was homogenized into poly lactic-co-glycolic acid/tricalcium phosphate (PLGA/TCP) to form an icaritin-releasing porous composite scaffold (PLGA/TCP/icaritin) by fine-spinning technology. In vitro, high performance liquid chromatography was used to determine the release of icaritin during degradation of PLGA/TCP/icaritin. The osteogenic effects of PLGA/TCP/icaritin were evaluated using rat bone marrow mesenchymal stem cells (BMSCs). In vivo, the osteogenic effect of PLGA/TCP/icaritin was determined within a bone tunnel after core decompression in SAON rabbits and angiography within scaffolds was examined in rabbit muscle pouch model. In vitro study confirmed the sustainable release of icaritin from PLGA/TCP/icaritin with the bioactive scaffold promoting the proliferation and osteoblastic differentiation of rat BMSCs. In vivo study showed that PLGA/TCP/icaritin significantly promoted new bone formation within the bone defect after core decompression in SAON rabbits and enhanced neovascularization in the rabbit muscle pouch experiment. In conclusion, PLGA/TCP/icaritin is an innovative local delivery system that demonstrates sustainable release of osteogenic phytomolecule icaritin enhancing bone repair in an SAON rabbit model. The supplement of scaffold materials with bioactive phytomolecule(s) might improve treatment efficiency in challenging orthopedic conditions.

Original languageEnglish
Pages (from-to)164-172
Number of pages9
JournalJournal of Orthopaedic Research
Volume31
Issue number1
DOIs
Publication statusPublished - Jan 2013

Scopus Subject Areas

  • Orthopedics and Sports Medicine

User-Defined Keywords

  • angiogenesis
  • osteogenesis
  • PLGA/TCP/icaritin
  • porous scaffold
  • SAON

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