Exogenous hydrogen sulphide alleviates nodule senescence in Glycine max-Sinorhizobium fredii symbiotic system

Juan Chen*, Wu Yu Liu, Xin Zhang, Qing Pan, Feng Shuya Wang, Shi Ming Wen, Jian Hua Zhang, Ge Hong Wei*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review


Aim: Hydrogen sulphide (H2S), as a signalling molecule, regulates the process of plant growth and development. H2S is involved in the establishment of the legume and rhizobial symbiotic relationship, but little is known about the role of H2S during nodule senescence. Method: We studied the function of H2S during nodule senescence in the Glycine max-Sinorhizobium fredii symbiotic system. Physiological and biochemical methods were used to modulate nitrogen fixation, nodule structure, senescence-related protein and gene expression. Results: Our results indicated that H2S decreased the senescent nodule number, increased nitrogenase (Nase) activity and ureide content, and further promoted the accumulation of NO3-N, NH4+-N, and total N, which caused the accumulation of biomass in the late steps of symbiosis. H2S alleviated nodule senescence by maintaining the normal nitrogen fixation structure of nodules. Meanwhile, the expression levels of the NifH and NodGS proteins and the N metabolic enzyme activities were enhanced by H2S during nodule senescence. Moreover, H2S stimulated the expression of nitrogen metabolism-related genes in the nodules. Finally, senescence-related gene expression levels were regulated by H2S during nodule senescence. Conclusion: These findings suggested that H2S prolonged the lifespan of N2 fixation by delaying nodule senescence, which will be important for increasing soybean yield and agricultural sustainability development.

Original languageEnglish
Pages (from-to)603-623
Number of pages21
JournalPlant and Soil
Issue number1-2
Early online date31 Mar 2023
Publication statusPublished - Jul 2023

Scopus Subject Areas

  • Soil Science
  • Plant Science

User-Defined Keywords

  • Hydrogen sulphide (HS)
  • Nodule senescence
  • Senescence related genes
  • Soybean (Glycine max)
  • Symbiotic nitrogen fixation


Dive into the research topics of 'Exogenous hydrogen sulphide alleviates nodule senescence in Glycine max-Sinorhizobium fredii symbiotic system'. Together they form a unique fingerprint.

Cite this