TY - JOUR
T1 - Exogenous hydrogen sulphide alleviates nodule senescence in Glycine max-Sinorhizobium fredii symbiotic system
AU - Chen, Juan
AU - Liu, Wu Yu
AU - Zhang, Xin
AU - Pan, Qing
AU - Wang, Feng Shuya
AU - Wen, Shi Ming
AU - Zhang, Jian Hua
AU - Wei, Ge Hong
N1 - This study was financially supported by the Natural Science Foundation of China (NSFC) (U21A2029, 42177329), and the Hong Kong Research Grant Council (AoE/M-403/16).
Publisher Copyright:
© 2023, The Author(s), under exclusive licence to Springer Nature Switzerland AG.
PY - 2023/7
Y1 - 2023/7
N2 - Aim: Hydrogen sulphide (H2S), as a signalling molecule, regulates the process of plant growth and development. H2S is involved in the establishment of the legume and rhizobial symbiotic relationship, but little is known about the role of H2S during nodule senescence. Method: We studied the function of H2S during nodule senescence in the Glycine max-Sinorhizobium fredii symbiotic system. Physiological and biochemical methods were used to modulate nitrogen fixation, nodule structure, senescence-related protein and gene expression. Results: Our results indicated that H2S decreased the senescent nodule number, increased nitrogenase (Nase) activity and ureide content, and further promoted the accumulation of NO3−-N, NH4+-N, and total N, which caused the accumulation of biomass in the late steps of symbiosis. H2S alleviated nodule senescence by maintaining the normal nitrogen fixation structure of nodules. Meanwhile, the expression levels of the NifH and NodGS proteins and the N metabolic enzyme activities were enhanced by H2S during nodule senescence. Moreover, H2S stimulated the expression of nitrogen metabolism-related genes in the nodules. Finally, senescence-related gene expression levels were regulated by H2S during nodule senescence. Conclusion: These findings suggested that H2S prolonged the lifespan of N2 fixation by delaying nodule senescence, which will be important for increasing soybean yield and agricultural sustainability development.
AB - Aim: Hydrogen sulphide (H2S), as a signalling molecule, regulates the process of plant growth and development. H2S is involved in the establishment of the legume and rhizobial symbiotic relationship, but little is known about the role of H2S during nodule senescence. Method: We studied the function of H2S during nodule senescence in the Glycine max-Sinorhizobium fredii symbiotic system. Physiological and biochemical methods were used to modulate nitrogen fixation, nodule structure, senescence-related protein and gene expression. Results: Our results indicated that H2S decreased the senescent nodule number, increased nitrogenase (Nase) activity and ureide content, and further promoted the accumulation of NO3−-N, NH4+-N, and total N, which caused the accumulation of biomass in the late steps of symbiosis. H2S alleviated nodule senescence by maintaining the normal nitrogen fixation structure of nodules. Meanwhile, the expression levels of the NifH and NodGS proteins and the N metabolic enzyme activities were enhanced by H2S during nodule senescence. Moreover, H2S stimulated the expression of nitrogen metabolism-related genes in the nodules. Finally, senescence-related gene expression levels were regulated by H2S during nodule senescence. Conclusion: These findings suggested that H2S prolonged the lifespan of N2 fixation by delaying nodule senescence, which will be important for increasing soybean yield and agricultural sustainability development.
KW - Hydrogen sulphide (HS)
KW - Nodule senescence
KW - Senescence related genes
KW - Soybean (Glycine max)
KW - Symbiotic nitrogen fixation
UR - http://www.scopus.com/inward/record.url?scp=85151349172&partnerID=8YFLogxK
U2 - 10.1007/s11104-023-05997-6
DO - 10.1007/s11104-023-05997-6
M3 - Journal article
AN - SCOPUS:85151349172
SN - 0032-079X
VL - 488
SP - 603
EP - 623
JO - Plant and Soil
JF - Plant and Soil
IS - 1-2
ER -