Ex Vivo and in Situ Evaluation of 'Dispelling-Wind' Chinese Medicine Herb-Drugs on Intestinal Absorption of Chlorogenic Acid

Lixiang Zhai, Jun Shi*, Weitong Xu, Michael Heinrich, Jianying Wang, Wenji Deng

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

18 Citations (Scopus)

Abstract

This study aims to investigate the additive or synergistic effects and mechanism of intestinal absorption of extracts from two commonly used 'dispelling-wind' TCM botanical drugs [roots of Angelica dahurica (Hoffm.) Benth. & Hook. f. ex Franch. & Sav. (RAD) and Saposhnikovia divaricata (Turcz.) Schischk. (RSD)] using chlorogenic acid as a marker substance. Ex vivo everted intestinal sac and in situ single pass perfusion methods using rats were employed to investigate the effects of two TCM botanical drugs extracts on the intestinal absorption of chlorogenic acid. Both the extracts of RAD and RSD showed synergistic properties on the intestinal absorption of chlorogenic acid. The verapamil (a P-gp inhibitor) and intestinal dysbacteriosis model induced by norfloxacin increased the Papp and Ka of intestinal absorption of chlorogenic acid. These synergistic effects on intestinal absorption in a rat model can be correlated with the inhibition of P-gp and regulation of gut microbiota. This experimental approach has helped to better understand changes in the absorption of chlorogenic acid under different conditions.

Original languageEnglish
Pages (from-to)1974-1981
Number of pages8
JournalPhytotherapy Research
Volume29
Issue number12
Early online date30 Oct 2015
DOIs
Publication statusPublished - Dec 2015

Scopus Subject Areas

  • Pharmacology

User-Defined Keywords

  • Angelica dahurica
  • chlorogenic acid
  • everted rat gut sac
  • intestinal absorption
  • Saposhnikovia divaricata
  • single-pass intestinal perfusion

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