Evolutionary mode of metallothioneins inferred from Cd-MT genes of tetrahymena

Ming Liang Wan*, Wei Song Feng, Wei Miao, Ming Hung WONG

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

2 Citations (Scopus)


From Tetrahymena thermophila (strain BF5), the coding region of Cd-MT gene was cloned and sequenced, and identified as MTT1 isoform. A serial duplicate structure is discovered in its amino acid sequence, which separates the coding region into three parts (Part 1: 7-61; Part 2: 64-118; Part 3: 122-162). The alignments among them and comparison with the corresponding parts of MT1 isoform suggest that MT1 and MTT1 isoforms both come from the same ancient gene that is homologous to Part 1, and Cd-MTs of Tetrahymena are aroused by such ancient gene duplication. The prediction of secondary structures and the analysis of the disulfide-bonding state of cysteine show that there are a lot of differences between MT1 and MTT1 isoforms, which maybe relate to their function mechanism.

Original languageEnglish
Pages (from-to)925-930
Number of pages6
JournalWuhan University Journal of Natural Sciences
Issue number5
Publication statusPublished - Sept 2005

Scopus Subject Areas

  • General

User-Defined Keywords

  • Evolutionary mode
  • Gene duplication
  • Metal-binding sites
  • Metallothioneins
  • Protein spatial structure
  • Tetrahymena thermophila

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