TY - JOUR
T1 - Evaluation of bisphenol A exposure induced oxidative RNA damage by liquid chromatography-mass spectrometry
AU - Li, Shangfu
AU - Jin, Yibao
AU - Zhao, Hongzhi
AU - Jiang, Yuyang
AU - Cai, Zongwei
N1 - Funding Information:
This work was supported by National Natural Science Foundation of China ( NSFC 21505111 and NSFC 21437002 ), General Research Fund from the Research Grants Council of the Hong Kong Special Administrative Region, China ( No. 12301915 and No. 12319716 ) and Shenzhen Fundamental Research Program ( JCYJ20160530173520985 ). Shangfu Li would like to thank the Hong Kong PhD Fellowship Scheme from Hong Kong Research Grant Council, as well as supporting from Mr. Kwok Yat Wai and Madam Kwok Chung Bo Fun Graduate School Development Fund.
PY - 2019/5
Y1 - 2019/5
N2 - Highlighted evidence suggests the possible implication of bisphenol A (BPA) exposure on a variety of biological functions, such as DNA damage. Similar to DNA, exposed to BPA may also have potential risks to RNA damage due to its induction of reactive oxygen species. However, there are no related research reports about such health risks of BPA. Therefore, this work tried to investigate the BPA exposure induced oxidative RNA damage by detecting urinary nucleosides, the end-products of RNA metabolism. An ultra-high performance liquid chromatography-Orbitrap mass spectrometry method was applied to selectively and sensitively determine urinary nucleosides. As a result, 66 nucleosides were identified and the effects of BPA exposure on these nucleosides in rat urine samples were evaluated. The nucleosides showed different changing tendency along with different exposure dose of BPA. The strongest effect was observed in high does-exposure rats, indicating dose-response relationship between BPA-treatment and urinary nucleosides. Significant change of some nucleosides, including 8-oxoguanosine, was observed in the high-dose exposure group, suggesting obvious RNA damage to rats. To the best of our knowledge, it is the first study about the RNA damage induced by BPA exposure. The results provided a new perspective on the toxic effects of BPA exposure.
AB - Highlighted evidence suggests the possible implication of bisphenol A (BPA) exposure on a variety of biological functions, such as DNA damage. Similar to DNA, exposed to BPA may also have potential risks to RNA damage due to its induction of reactive oxygen species. However, there are no related research reports about such health risks of BPA. Therefore, this work tried to investigate the BPA exposure induced oxidative RNA damage by detecting urinary nucleosides, the end-products of RNA metabolism. An ultra-high performance liquid chromatography-Orbitrap mass spectrometry method was applied to selectively and sensitively determine urinary nucleosides. As a result, 66 nucleosides were identified and the effects of BPA exposure on these nucleosides in rat urine samples were evaluated. The nucleosides showed different changing tendency along with different exposure dose of BPA. The strongest effect was observed in high does-exposure rats, indicating dose-response relationship between BPA-treatment and urinary nucleosides. Significant change of some nucleosides, including 8-oxoguanosine, was observed in the high-dose exposure group, suggesting obvious RNA damage to rats. To the best of our knowledge, it is the first study about the RNA damage induced by BPA exposure. The results provided a new perspective on the toxic effects of BPA exposure.
KW - Bisphenol A
KW - Liquid chromatography-mass spectrometry
KW - Modified nucleosides
KW - Parallel reaction monitoring
KW - RNA damage
KW - Toxicity
UR - http://www.scopus.com/inward/record.url?scp=85060905088&partnerID=8YFLogxK
U2 - 10.1016/j.chemosphere.2019.01.120
DO - 10.1016/j.chemosphere.2019.01.120
M3 - Journal article
C2 - 30708157
AN - SCOPUS:85060905088
SN - 0045-6535
VL - 222
SP - 235
EP - 242
JO - Chemosphere
JF - Chemosphere
ER -
