TY - JOUR
T1 - Euphorbia factor L2 induces apoptosis in A549 cells through the mitochondrial pathway
AU - Lin, Minting
AU - Tang, Sili
AU - Zhang, Chao
AU - CHEN, Hubiao
AU - Huang, Wenjing
AU - Liu, Yun
AU - Zhang, Jianye
N1 - Funding Information:
The work was supported by National Natural Science Foundation of China (No. 81473320), Fund from Guangdong Science and Technology Department & Guangdong Academy of Traditional Chinese Medicine (2016A020226024), Fund of Guangdong Education Department (2015KTSCX112), the Science Fund of the Education Bureau of Guangzhou City (1201410039 and 2012C208).
PY - 2017/1/1
Y1 - 2017/1/1
N2 - Euphorbia factor L2, a lathyrane diterpenoid isolated from caper euphorbia seed (the seeds of Euphorbia lathyris L.), has been traditionally applied to treat cancer. This article focuses on the cytotoxic activity of Euphorbia factor L2 against lung carcinoma A549 cells and the mechanism by which apoptosis is induced. We analyzed the cytotoxicity and related mechanism of Euphorbia factor L2 with an MTT assay, an annexin V-FITC/PI test, a colorimetric assay, and immunoblotting. Euphorbia factor L2 showed potent cytotoxicity to A549 cells. Euphorbia factor L2 led to an increase in reactive oxygen species (ROS) generation, a loss of mitochondrial electrochemical potential, release of cytochrome c, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase, suggesting that Euphorbia factor L2 induced apoptosis through a mitochondrial pathway. The cytotoxic activity of Euphorbia factor L2 in A549 cells and the related mechanisms of apoptotic induction provide support for the further investigation of caper euphorbia seeds.
AB - Euphorbia factor L2, a lathyrane diterpenoid isolated from caper euphorbia seed (the seeds of Euphorbia lathyris L.), has been traditionally applied to treat cancer. This article focuses on the cytotoxic activity of Euphorbia factor L2 against lung carcinoma A549 cells and the mechanism by which apoptosis is induced. We analyzed the cytotoxicity and related mechanism of Euphorbia factor L2 with an MTT assay, an annexin V-FITC/PI test, a colorimetric assay, and immunoblotting. Euphorbia factor L2 showed potent cytotoxicity to A549 cells. Euphorbia factor L2 led to an increase in reactive oxygen species (ROS) generation, a loss of mitochondrial electrochemical potential, release of cytochrome c, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase, suggesting that Euphorbia factor L2 induced apoptosis through a mitochondrial pathway. The cytotoxic activity of Euphorbia factor L2 in A549 cells and the related mechanisms of apoptotic induction provide support for the further investigation of caper euphorbia seeds.
KW - Anticancer agent
KW - Apoptosis
KW - Caper euphorbia seed
KW - Euphorbia Factor L2
KW - Euphorbia lathyris L.
KW - Mitochondrial pathway
UR - http://www.scopus.com/inward/record.url?scp=84997724747&partnerID=8YFLogxK
U2 - 10.1016/j.apsb.2016.06.008
DO - 10.1016/j.apsb.2016.06.008
M3 - Journal article
AN - SCOPUS:84997724747
SN - 2211-3835
VL - 7
SP - 59
EP - 64
JO - Acta Pharmaceutica Sinica B
JF - Acta Pharmaceutica Sinica B
IS - 1
ER -
