Euphorbia factor L1 reverses ABCB1-mediated multidrug resistance involving interaction with ABCB1 independent of ABCB1 downregualtion

Jian Ye Zhang, Yan Jun Mi, Shu Peng Chen, Fang Wang, Yong Ju Liang, Li Sheng Zheng, Cheng Jun Shi, Li Yang Tao, Li Ming Chen, Hubiao CHEN, Li Wu Fu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

Euphorbia factor L1 (EFL1) belongs to diterpenoids of genus Euphorbia. In this article, its reversal activity against ABCB1-mediated MDR in KBv200 and MCF-7/adr cells was reported. However, EFL1 did not alter the sensitivity of KB and MCF-7 cells to chemotherapeutic agents. Meanwhile, EFL1 significantly increased accumulation of doxorubicin and rhodamine 123 in KBv200 and MCF-7/adr cells, showing no siginficant influence on that of KB and MCF-7 cells. Furthermore, EFL1 could enhance the ATP hydrolysis activity of ABCB1 stimulated by verapamil. At the same time, EFL1 inhibited the efflux of ABCB1 in KBv200 and MCF-7/adr cells. In addition, EFL1 did not downregulate expression of ABCB1 in KBv200 and MCF-7/adr cells either in mRNA or protein level.

Original languageEnglish
Pages (from-to)1076-1083
Number of pages8
JournalJournal of Cellular Biochemistry
Volume112
Issue number4
DOIs
Publication statusPublished - Apr 2011

Scopus Subject Areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

User-Defined Keywords

  • ABCB1
  • Euphorbia factor L1
  • Multidrug resistance

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