Euphorbia factor L1 reverses ABCB1-mediated multidrug resistance involving interaction with ABCB1 independent of ABCB1 downregualtion

Jian Ye Zhang; Yan Jun Mi; Shu Peng Chen; Fang Wang; Yong Ju Liang; Li Sheng Zheng; Cheng Jun Shi; Li Yang Tao; Li Ming Chen; Hubiao Chen; Li Wu Fu

doi:10.1002/jcb.23021

Euphorbia factor L1 reverses ABCB1-mediated multidrug resistance involving interaction with ABCB1 independent of ABCB1 downregualtion

Jian Ye Zhang, Yan Jun Mi, Shu Peng Chen, Fang Wang, Yong Ju Liang, Li Sheng Zheng, Cheng Jun Shi, Li Yang Tao, Li Ming Chen, Hubiao Chen, Li Wu Fu^*

^*Corresponding author for this work

School of Chinese Medicine

Research output: Contribution to journal › Journal article › peer-review

23 Citations (Scopus)

Abstract

Euphorbia factor L1 (EFL1) belongs to diterpenoids of genus Euphorbia. In this article, its reversal activity against ABCB1-mediated MDR in KBv200 and MCF-7/adr cells was reported. However, EFL1 did not alter the sensitivity of KB and MCF-7 cells to chemotherapeutic agents. Meanwhile, EFL1 significantly increased accumulation of doxorubicin and rhodamine 123 in KBv200 and MCF-7/adr cells, showing no siginficant influence on that of KB and MCF-7 cells. Furthermore, EFL1 could enhance the ATP hydrolysis activity of ABCB1 stimulated by verapamil. At the same time, EFL1 inhibited the efflux of ABCB1 in KBv200 and MCF-7/adr cells. In addition, EFL1 did not downregulate expression of ABCB1 in KBv200 and MCF-7/adr cells either in mRNA or protein level.

Original language	English
Pages (from-to)	1076-1083
Number of pages	8
Journal	Journal of Cellular Biochemistry
Volume	112
Issue number	4
DOIs	https://doi.org/10.1002/jcb.23021
Publication status	Published - Apr 2011

Scopus Subject Areas

Biochemistry
Molecular Biology
Cell Biology

User-Defined Keywords

ABCB1
Euphorbia factor L1
Multidrug resistance

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10.1002/jcb.23021

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@article{ce7ab0cb65eb41b1ae5057dccc5dcce8,

title = "Euphorbia factor L1 reverses ABCB1-mediated multidrug resistance involving interaction with ABCB1 independent of ABCB1 downregualtion",

abstract = "Euphorbia factor L1 (EFL1) belongs to diterpenoids of genus Euphorbia. In this article, its reversal activity against ABCB1-mediated MDR in KBv200 and MCF-7/adr cells was reported. However, EFL1 did not alter the sensitivity of KB and MCF-7 cells to chemotherapeutic agents. Meanwhile, EFL1 significantly increased accumulation of doxorubicin and rhodamine 123 in KBv200 and MCF-7/adr cells, showing no siginficant influence on that of KB and MCF-7 cells. Furthermore, EFL1 could enhance the ATP hydrolysis activity of ABCB1 stimulated by verapamil. At the same time, EFL1 inhibited the efflux of ABCB1 in KBv200 and MCF-7/adr cells. In addition, EFL1 did not downregulate expression of ABCB1 in KBv200 and MCF-7/adr cells either in mRNA or protein level.",

keywords = "ABCB1, Euphorbia factor L1, Multidrug resistance",

author = "Zhang, {Jian Ye} and Mi, {Yan Jun} and Chen, {Shu Peng} and Fang Wang and Liang, {Yong Ju} and Zheng, {Li Sheng} and Shi, {Cheng Jun} and Tao, {Li Yang} and Chen, {Li Ming} and Hubiao Chen and Fu, {Li Wu}",

year = "2011",

month = apr,

doi = "10.1002/jcb.23021",

language = "English",

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pages = "1076--1083",

journal = "Journal of Cellular Biochemistry",

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T1 - Euphorbia factor L1 reverses ABCB1-mediated multidrug resistance involving interaction with ABCB1 independent of ABCB1 downregualtion

AU - Zhang, Jian Ye

AU - Mi, Yan Jun

AU - Chen, Shu Peng

AU - Wang, Fang

AU - Liang, Yong Ju

AU - Zheng, Li Sheng

AU - Shi, Cheng Jun

AU - Tao, Li Yang

AU - Chen, Li Ming

AU - Chen, Hubiao

AU - Fu, Li Wu

PY - 2011/4

Y1 - 2011/4

N2 - Euphorbia factor L1 (EFL1) belongs to diterpenoids of genus Euphorbia. In this article, its reversal activity against ABCB1-mediated MDR in KBv200 and MCF-7/adr cells was reported. However, EFL1 did not alter the sensitivity of KB and MCF-7 cells to chemotherapeutic agents. Meanwhile, EFL1 significantly increased accumulation of doxorubicin and rhodamine 123 in KBv200 and MCF-7/adr cells, showing no siginficant influence on that of KB and MCF-7 cells. Furthermore, EFL1 could enhance the ATP hydrolysis activity of ABCB1 stimulated by verapamil. At the same time, EFL1 inhibited the efflux of ABCB1 in KBv200 and MCF-7/adr cells. In addition, EFL1 did not downregulate expression of ABCB1 in KBv200 and MCF-7/adr cells either in mRNA or protein level.

AB - Euphorbia factor L1 (EFL1) belongs to diterpenoids of genus Euphorbia. In this article, its reversal activity against ABCB1-mediated MDR in KBv200 and MCF-7/adr cells was reported. However, EFL1 did not alter the sensitivity of KB and MCF-7 cells to chemotherapeutic agents. Meanwhile, EFL1 significantly increased accumulation of doxorubicin and rhodamine 123 in KBv200 and MCF-7/adr cells, showing no siginficant influence on that of KB and MCF-7 cells. Furthermore, EFL1 could enhance the ATP hydrolysis activity of ABCB1 stimulated by verapamil. At the same time, EFL1 inhibited the efflux of ABCB1 in KBv200 and MCF-7/adr cells. In addition, EFL1 did not downregulate expression of ABCB1 in KBv200 and MCF-7/adr cells either in mRNA or protein level.

KW - ABCB1

KW - Euphorbia factor L1

KW - Multidrug resistance

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SN - 0730-2312

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SP - 1076

EP - 1083

JO - Journal of Cellular Biochemistry

JF - Journal of Cellular Biochemistry

IS - 4

ER -

Euphorbia factor L1 reverses ABCB1-mediated multidrug resistance involving interaction with ABCB1 independent of ABCB1 downregualtion

Abstract

Scopus Subject Areas

User-Defined Keywords

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