Ethyl acetate fraction of the root of Rubia cordifolia L. inhibits keratinocyte proliferation in vitro and promotes keratinocyte differentiation in vivo: Potential application for psoriasis treatment

Z. X. Lin, B. W. Jiao, C. T. Che, Z. Zuo, C. F. Mok, M. Zhao, W. K.K. Ho, W. P. Tse, K. Y. Lam, R. Q. Fan, Zhijun YANG, C. H.K. Cheng

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

Psoriasis is a skin disease associated with hyperproliferation and aberrant differentiation of keratinocytes. Our previous studies have identified the root of Rubia cordifolia L. as a potent antiproliferative and apoptogenic agent in cultured HaCaT cells (IC50 1.4 μg/ml). In the present study, ethanolic extract of Radix Rubiae was fractioned sequentially with hexane, ethyl acetate (EA), n-butanol and water. EA fraction was found to possess most potent antiproliferative action on HaCaT cells (IC50 0.9 μg/ml). Mechanistic study revealed that EA fraction induced apoptosis on HaCaT cells, as it was capable of inducing apoptotic morphological changes. Annexin V-PI staining assay also demonstrated that EA fraction significantly augmented HaCaT apoptosis. In addition, EA fraction decreased mitochondrial membrane potential in a concentration- and time-dependent manner. The standardized EA fraction was formulated into topical gel and its keratinocyte-modulating action was tested on mouse tail model. EA fraction dose-dependently increased the number and thickness of granular layer and epidermal thickness on mouse tail skin, indicative of the keratinocyte differentiation-inducing activity. Taking the in vitro and in vivo findings together, the present preclinical study confirms that EA fraction is a promising antipsoriatic agent warranting further development for psoriasis treatment.

Original languageEnglish
Pages (from-to)1056-1064
Number of pages9
JournalPhytotherapy Research
Volume24
Issue number7
DOIs
Publication statusPublished - Jul 2010

Scopus Subject Areas

  • Pharmacology

User-Defined Keywords

  • Apoptosis
  • Differentiation
  • HaCaT keratinocytes
  • Mouse tail test
  • Proliferation
  • Psoriasis
  • Rubia cordifolia L.

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