Establishment of HPLC-DAD-MS fingerprint of fresh Houttuynia cordata

Jiang Meng; Kelvin Sze-Yin Leung; Zhihong Jiang; Xiaoping Dong; Zhongzhen Zhao; Li-Jia Xu

doi:10.1248/cpb.53.1604

Establishment of HPLC-DAD-MS fingerprint of fresh Houttuynia cordata

Jiang Meng, Kelvin Sze-Yin Leung, Zhihong Jiang, Xiaoping Dong, Zhongzhen Zhao^*, Li-Jia Xu

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › peer-review

35 Citations (Scopus)

Abstract

A HPLC-DAD-MS fingerprint method of fresh Houttuynia cordata THUNB. was developed basing on the consistent chromatographic features among 11 batches of authentic samples. Major chemical components including phenolic compounds, flavones and alkaloids were simultaneously analyzed. Eleven common peaks in the fingerprint were chosen and identified by comparing their UV and ESI-MS data with the authentic compounds. The unique properties of this HPLC-DAD-MS fingerprint were successfully applied to analyze and differentiate samples from different geographical origins, processing methods and various medicinal parts of H. cordata. The results showed that these variations will give rise to differences in identities and/or abundance of chemical compounds, indicating that a comprehensive quality evaluation of those major ingredients in H. cordata is critical to assess and represent its overall quality.

Original language	English
Pages (from-to)	1604-1609
Number of pages	6
Journal	Chemical and Pharmaceutical Bulletin
Volume	53
Issue number	12
DOIs	https://doi.org/10.1248/cpb.53.1604
Publication status	Published - Dec 2005

Scopus Subject Areas

Chemistry(all)
Drug Discovery

User-Defined Keywords

Fingerprint
Houttuynia cordata
HPLC-DAD-MS
Quality control

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10.1248/cpb.53.1604

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title = "Establishment of HPLC-DAD-MS fingerprint of fresh Houttuynia cordata",

abstract = "A HPLC-DAD-MS fingerprint method of fresh Houttuynia cordata THUNB. was developed basing on the consistent chromatographic features among 11 batches of authentic samples. Major chemical components including phenolic compounds, flavones and alkaloids were simultaneously analyzed. Eleven common peaks in the fingerprint were chosen and identified by comparing their UV and ESI-MS data with the authentic compounds. The unique properties of this HPLC-DAD-MS fingerprint were successfully applied to analyze and differentiate samples from different geographical origins, processing methods and various medicinal parts of H. cordata. The results showed that these variations will give rise to differences in identities and/or abundance of chemical compounds, indicating that a comprehensive quality evaluation of those major ingredients in H. cordata is critical to assess and represent its overall quality.",

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author = "Jiang Meng and Leung, {Kelvin Sze-Yin} and Zhihong Jiang and Xiaoping Dong and Zhongzhen Zhao and Li-Jia Xu",

note = "Funding Information: This work was supported by the Faculty Research Grant, Hong Kong Baptist University (FRG/03-04/II-39). The authors would like to thank Chi-Leung Chan, School of Chinese medicine, Hong Kong Baptist University, for the technical support in the operation of LC-MS. Publisher copyright: {\textcopyright} 2005 Pharmaceutical Society of Japan",

year = "2005",

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doi = "10.1248/cpb.53.1604",

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AU - Leung, Kelvin Sze-Yin

AU - Jiang, Zhihong

AU - Dong, Xiaoping

AU - Zhao, Zhongzhen

AU - Xu, Li-Jia

N1 - Funding Information: This work was supported by the Faculty Research Grant, Hong Kong Baptist University (FRG/03-04/II-39). The authors would like to thank Chi-Leung Chan, School of Chinese medicine, Hong Kong Baptist University, for the technical support in the operation of LC-MS. Publisher copyright: © 2005 Pharmaceutical Society of Japan

PY - 2005/12

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N2 - A HPLC-DAD-MS fingerprint method of fresh Houttuynia cordata THUNB. was developed basing on the consistent chromatographic features among 11 batches of authentic samples. Major chemical components including phenolic compounds, flavones and alkaloids were simultaneously analyzed. Eleven common peaks in the fingerprint were chosen and identified by comparing their UV and ESI-MS data with the authentic compounds. The unique properties of this HPLC-DAD-MS fingerprint were successfully applied to analyze and differentiate samples from different geographical origins, processing methods and various medicinal parts of H. cordata. The results showed that these variations will give rise to differences in identities and/or abundance of chemical compounds, indicating that a comprehensive quality evaluation of those major ingredients in H. cordata is critical to assess and represent its overall quality.

AB - A HPLC-DAD-MS fingerprint method of fresh Houttuynia cordata THUNB. was developed basing on the consistent chromatographic features among 11 batches of authentic samples. Major chemical components including phenolic compounds, flavones and alkaloids were simultaneously analyzed. Eleven common peaks in the fingerprint were chosen and identified by comparing their UV and ESI-MS data with the authentic compounds. The unique properties of this HPLC-DAD-MS fingerprint were successfully applied to analyze and differentiate samples from different geographical origins, processing methods and various medicinal parts of H. cordata. The results showed that these variations will give rise to differences in identities and/or abundance of chemical compounds, indicating that a comprehensive quality evaluation of those major ingredients in H. cordata is critical to assess and represent its overall quality.

KW - Fingerprint

KW - Houttuynia cordata

KW - HPLC-DAD-MS

KW - Quality control

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JF - Chemical and Pharmaceutical Bulletin

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ER -

Establishment of HPLC-DAD-MS fingerprint of fresh Houttuynia cordata

Abstract

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