Eruberin A, a natural flavanol glycoside, exerts anti-fibrotic action on pancreatic stellate cells

Siu Wai TSANG*, Hongjie ZHANG, Ye Gao Chen, Kathy K W AU YEUNG, Zhaoxiang BIAN

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)
1 Downloads (Pure)

Abstract

Background: Eruberin A (2, 3-dehydroflavonoid), a flavanol glycoside isolated from Pronephrium penangianum, has been used as a blood-nourishing folk medicine for centuries; however, it indeed possesses a variety of other health-promoting benefits including antifibrotic bioactivity. Activation of pancreatic stellate cells (PSCs) is the key initiating step in pancreatic fibrosis, which is a characteristic feature associated with chronic pancreatitis and pancreatic adenocarcinoma. Methods: The anti-fibrotic effect of eruberin A and the underlying mechanisms of its anti-fibrotic action in LTC-14 cells, which retained essential characteristics and morphological features of primary PSCs, were examined by means of real-time polymerase chain reactions, Western blotting and immunostaining. Results: The application of eruberin A (20 μg/ml) effectively inhibited the expression levels of fibrotic mediators namely alphasmooth muscle actin, fibronectin and type I-collagen, so as the sonic hedgehog signaling pathway components post transforming growth factor-beta (5 ng/ml) stimulation. Eruberin A treatment also led to a notable decrease in the activation of nuclear factor-kappaB (NF-κB) and the phosphorylation of phosphoinositide 3-kinase (PI3K)/serine-threonine kinase (AKT). Conclusion: Our results demonstrated that eruberin A significantly suppressed the expression levels of fibrotic mediators in PSCs, and we suggest that its anti-fibrotic mechanism was associated with an attenuation of the PI3K/AKT/NF-κB signaling pathway.

Original languageEnglish
Pages (from-to)2433-2446
Number of pages14
JournalCellular Physiology and Biochemistry
Volume36
Issue number6
DOIs
Publication statusPublished - 20 Aug 2015

Scopus Subject Areas

  • Physiology

User-Defined Keywords

  • Alpha-smooth muscle actin
  • Nuclear factor-kappaB
  • Pancreatic fibrosis
  • Pancreatic stellate cells
  • PI3K/AKT
  • Sonic hedgehog

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