TY - JOUR
T1 - Equipment-free, gold nanoparticle based semiquantitative assay of SARS-CoV-2-S1RBD IgG from fingertip blood
T2 - A practical strategy for on-site measurement of COVID-19 antibodies
AU - Zhu, Zhitong
AU - Ma, Xiaoming
AU - Zhu, Lin
AU - Luo, Qiong
AU - Lin, Nan
AU - Chen, Zhonghui
AU - Zhao, Xingchen
AU - Lin, Zhenyu
AU - Cai, Zongwei
N1 - Funding Information:
This work was supported by the Innovation and Technology Fund ( SST/067/20GP ) of Hong Kong SAR, China, and donation Kwok Chung Bo Fun Charitable Fund for the establishment of the Kwok Yat Wai Endowed Chair of Environmental and Biological Analysis.
Publisher Copyright:
© 2022 Elsevier B.V.
PY - 2022/8/15
Y1 - 2022/8/15
N2 - COVID-19 remains as a major threat to human society. A reliable, sensitive, rapid, and low requirement assay for serum neutralizing antibodies is needed as a pandemic management tool for estimation of revaccination time and implementation of “immune passport”. Using gold nanoparticle (AuNR) as an immunosensor, we have established a semi-quantitative, instrument-free assay for measuring antibody level against SRAS-CoV-2 spike1 (S1) receptor binding domain (RBD) from fingertip blood samples. The testing results by the developed method correlated well with those obtained from conventional ELISA assay, indicating reliable quantitation could be achieved without use of plate reader. A declined of immunoglobulin G (IgG) antibody associated with vaccination time was observed, which agreed well with the data from other reports. The developed method provides a potentially complementary strategy for on-site measurement of COVID-19 antibodies.
AB - COVID-19 remains as a major threat to human society. A reliable, sensitive, rapid, and low requirement assay for serum neutralizing antibodies is needed as a pandemic management tool for estimation of revaccination time and implementation of “immune passport”. Using gold nanoparticle (AuNR) as an immunosensor, we have established a semi-quantitative, instrument-free assay for measuring antibody level against SRAS-CoV-2 spike1 (S1) receptor binding domain (RBD) from fingertip blood samples. The testing results by the developed method correlated well with those obtained from conventional ELISA assay, indicating reliable quantitation could be achieved without use of plate reader. A declined of immunoglobulin G (IgG) antibody associated with vaccination time was observed, which agreed well with the data from other reports. The developed method provides a potentially complementary strategy for on-site measurement of COVID-19 antibodies.
KW - AuNR
KW - Colorimetric reagent
KW - Fingertip blood
KW - IgG antibody
KW - SARS-CoV-2
UR - http://www.scopus.com/inward/record.url?scp=85129077240&partnerID=8YFLogxK
U2 - 10.1016/j.talanta.2022.123498
DO - 10.1016/j.talanta.2022.123498
M3 - Journal article
C2 - 35489095
AN - SCOPUS:85129077240
SN - 0039-9140
VL - 246
JO - Talanta
JF - Talanta
M1 - 123498
ER -