Equipment-free, gold nanoparticle based semiquantitative assay of SARS-CoV-2-S1RBD IgG from fingertip blood: A practical strategy for on-site measurement of COVID-19 antibodies

Zhitong Zhu, Xiaoming Ma, Lin Zhu*, Qiong Luo, Nan Lin, Zhonghui Chen, Xingchen Zhao, Zhenyu Lin, Zongwei Cai

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

3 Citations (Scopus)

Abstract

COVID-19 remains as a major threat to human society. A reliable, sensitive, rapid, and low requirement assay for serum neutralizing antibodies is needed as a pandemic management tool for estimation of revaccination time and implementation of “immune passport”. Using gold nanoparticle (AuNR) as an immunosensor, we have established a semi-quantitative, instrument-free assay for measuring antibody level against SRAS-CoV-2 spike1 (S1) receptor binding domain (RBD) from fingertip blood samples. The testing results by the developed method correlated well with those obtained from conventional ELISA assay, indicating reliable quantitation could be achieved without use of plate reader. A declined of immunoglobulin G (IgG) antibody associated with vaccination time was observed, which agreed well with the data from other reports. The developed method provides a potentially complementary strategy for on-site measurement of COVID-19 antibodies.

Original languageEnglish
Article number123498
JournalTalanta
Volume246
DOIs
Publication statusPublished - 15 Aug 2022

Scopus Subject Areas

  • Analytical Chemistry

User-Defined Keywords

  • AuNR
  • Colorimetric reagent
  • Fingertip blood
  • IgG antibody
  • SARS-CoV-2

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