Epidermal growth factor induces Gadd45 (growth arrest and DNA damage inducible protein) expression in A431 cells

David W F Fong; Chung Hang Leung; Wing Lam; Nai Sum Wong; Shuk Han Cheng

doi:10.1016/S0167-4781(00)00287-6

Epidermal growth factor induces Gadd45 (growth arrest and DNA damage inducible protein) expression in A431 cells

David W F Fong^*, Chung Hang Leung, Wing Lam, Nai Sum Wong, Shuk Han Cheng

^*Corresponding author for this work

School of Chinese Medicine

Research output: Contribution to journal › Journal article › peer-review

12 Citations (Scopus)

Abstract

Epidermal growth factor (EGF) receptor over-expressing, p53-deficient A431 cells response to toxic dose of EGF by G1 arrest and apoptosis. Applying cDNA expression array technology we demonstrated that EGF increased the levels of Gadd45 mRNA. Northern blot and Western blot analyses confirmed that both Gadd45 mRNA and protein were increased. Concurrently half-lives of Gadd45 mRNA and protein also increased. Nuclear run-on experiments did not show a large increase of Gadd45 mRNA transcription rate. Gadd45 mRNA and protein started to increase after 1 h of EGF treatment and remained high for up to 10 h. We have also confirmed previous studies which showed that in EGF-stimulated A431 cells p21^Cip1/Waf1 (cyclin-dependent kinase interacting protein 1) was up-regulated within the same time frame. Thus it appears that in addition to inducing G2 arrest by directly disrupting Cdc2/Cyclin B1 complex in genotoxic-stressed cells, Gadd45 may also participate in G1 arrest in growth factor overexposed cells.

Original language	English
Pages (from-to)	250-256
Number of pages	7
Journal	Biochimica et Biophysica Acta - Gene Structure and Expression
Volume	1517
Issue number	2
DOIs	https://doi.org/10.1016/S0167-4781(00)00287-6
Publication status	Published - 26 Jan 2001

Scopus Subject Areas

Structural Biology
Biophysics
Biochemistry
Genetics

User-Defined Keywords

A431 cell
Apoptosis
Epidermal growth factor
Gadd45

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10.1016/S0167-4781(00)00287-6

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title = "Epidermal growth factor induces Gadd45 (growth arrest and DNA damage inducible protein) expression in A431 cells",

abstract = "Epidermal growth factor (EGF) receptor over-expressing, p53-deficient A431 cells response to toxic dose of EGF by G1 arrest and apoptosis. Applying cDNA expression array technology we demonstrated that EGF increased the levels of Gadd45 mRNA. Northern blot and Western blot analyses confirmed that both Gadd45 mRNA and protein were increased. Concurrently half-lives of Gadd45 mRNA and protein also increased. Nuclear run-on experiments did not show a large increase of Gadd45 mRNA transcription rate. Gadd45 mRNA and protein started to increase after 1 h of EGF treatment and remained high for up to 10 h. We have also confirmed previous studies which showed that in EGF-stimulated A431 cells p21Cip1/Waf1 (cyclin-dependent kinase interacting protein 1) was up-regulated within the same time frame. Thus it appears that in addition to inducing G2 arrest by directly disrupting Cdc2/Cyclin B1 complex in genotoxic-stressed cells, Gadd45 may also participate in G1 arrest in growth factor overexposed cells.",

keywords = "A431 cell, Apoptosis, Epidermal growth factor, Gadd45",

author = "Fong, {David W F} and Leung, {Chung Hang} and Wing Lam and Wong, {Nai Sum} and Cheng, {Shuk Han}",

note = "Funding Information: We thank Richard Y.C. Kong{\textquoteright}s research group for help with the Northern blotting. This work is supported by City University of Hong Kong Strategic Research Grant 7000864 and forms a part of C.H.L.{\textquoteright}s Ph.D. thesis.",

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AU - Fong, David W F

AU - Leung, Chung Hang

AU - Lam, Wing

AU - Wong, Nai Sum

AU - Cheng, Shuk Han

N1 - Funding Information: We thank Richard Y.C. Kong’s research group for help with the Northern blotting. This work is supported by City University of Hong Kong Strategic Research Grant 7000864 and forms a part of C.H.L.’s Ph.D. thesis.

PY - 2001/1/26

Y1 - 2001/1/26

N2 - Epidermal growth factor (EGF) receptor over-expressing, p53-deficient A431 cells response to toxic dose of EGF by G1 arrest and apoptosis. Applying cDNA expression array technology we demonstrated that EGF increased the levels of Gadd45 mRNA. Northern blot and Western blot analyses confirmed that both Gadd45 mRNA and protein were increased. Concurrently half-lives of Gadd45 mRNA and protein also increased. Nuclear run-on experiments did not show a large increase of Gadd45 mRNA transcription rate. Gadd45 mRNA and protein started to increase after 1 h of EGF treatment and remained high for up to 10 h. We have also confirmed previous studies which showed that in EGF-stimulated A431 cells p21Cip1/Waf1 (cyclin-dependent kinase interacting protein 1) was up-regulated within the same time frame. Thus it appears that in addition to inducing G2 arrest by directly disrupting Cdc2/Cyclin B1 complex in genotoxic-stressed cells, Gadd45 may also participate in G1 arrest in growth factor overexposed cells.

AB - Epidermal growth factor (EGF) receptor over-expressing, p53-deficient A431 cells response to toxic dose of EGF by G1 arrest and apoptosis. Applying cDNA expression array technology we demonstrated that EGF increased the levels of Gadd45 mRNA. Northern blot and Western blot analyses confirmed that both Gadd45 mRNA and protein were increased. Concurrently half-lives of Gadd45 mRNA and protein also increased. Nuclear run-on experiments did not show a large increase of Gadd45 mRNA transcription rate. Gadd45 mRNA and protein started to increase after 1 h of EGF treatment and remained high for up to 10 h. We have also confirmed previous studies which showed that in EGF-stimulated A431 cells p21Cip1/Waf1 (cyclin-dependent kinase interacting protein 1) was up-regulated within the same time frame. Thus it appears that in addition to inducing G2 arrest by directly disrupting Cdc2/Cyclin B1 complex in genotoxic-stressed cells, Gadd45 may also participate in G1 arrest in growth factor overexposed cells.

KW - A431 cell

KW - Apoptosis

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Epidermal growth factor induces Gadd45 (growth arrest and DNA damage inducible protein) expression in A431 cells

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