Enzyme-free quantification of exosomal microRNA by the target-triggered assembly of the polymer DNAzyme nanostructure

Dinggeng He; Luo Hai; Huizhen Wang; Ri Wu; Hung Wing Li

doi:10.1039/c7an01691c

Enzyme-free quantification of exosomal microRNA by the target-triggered assembly of the polymer DNAzyme nanostructure

Dinggeng He
, Luo Hai
, Huizhen Wang
, Ri Wu
, Hung Wing Li^*

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

28 Citations (Scopus)

Abstract

We herein report an efficient hybridization chain reaction (HCR)- and DNAzyme-based enzyme-free signal amplification for the detection of specific exosomal miRNAs in the culture medium of cancer cells and serum samples from cancer patients via the target-triggered self-assembly of the polymer DNAzyme nanostructure.

Original language	English
Pages (from-to)	813-816
Number of pages	4
Journal	Analyst
Volume	143
Issue number	4
Early online date	8 Jan 2018
DOIs	https://doi.org/10.1039/c7an01691c
Publication status	Published - 21 Feb 2018

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SDG 3 Good Health and Well-being

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10.1039/c7an01691c

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title = "Enzyme-free quantification of exosomal microRNA by the target-triggered assembly of the polymer DNAzyme nanostructure",

abstract = "We herein report an efficient hybridization chain reaction (HCR)- and DNAzyme-based enzyme-free signal amplification for the detection of specific exosomal miRNAs in the culture medium of cancer cells and serum samples from cancer patients via the target-triggered self-assembly of the polymer DNAzyme nanostructure.",

author = "Dinggeng He and Luo Hai and Huizhen Wang and Ri Wu and Li, \{Hung Wing\}",

note = "Funding Information: This work was supported in part by the Hong Kong Scholars Program, the National Natural Science Foundation of China (21775036), the Fundamental Research Funds for the Central Universities, the Research Foundation of Education Bureau of Hunan Province (15A023, 15A025), and the Hunan Provincial Natural Science Foundation (2016JJ4005, 2015JJ6010). We also acknowledge the support of the Research Grant Council of Hong Kong (12308416 and C2012-15G) and the research committee of Hong Kong Baptist University (FRG2/15-16/028).",

year = "2018",

month = feb,

day = "21",

doi = "10.1039/c7an01691c",

language = "English",

volume = "143",

pages = "813--816",

journal = "Analyst",

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TY - JOUR

T1 - Enzyme-free quantification of exosomal microRNA by the target-triggered assembly of the polymer DNAzyme nanostructure

AU - He, Dinggeng

AU - Hai, Luo

AU - Wang, Huizhen

AU - Wu, Ri

AU - Li, Hung Wing

N1 - Funding Information: This work was supported in part by the Hong Kong Scholars Program, the National Natural Science Foundation of China (21775036), the Fundamental Research Funds for the Central Universities, the Research Foundation of Education Bureau of Hunan Province (15A023, 15A025), and the Hunan Provincial Natural Science Foundation (2016JJ4005, 2015JJ6010). We also acknowledge the support of the Research Grant Council of Hong Kong (12308416 and C2012-15G) and the research committee of Hong Kong Baptist University (FRG2/15-16/028).

PY - 2018/2/21

Y1 - 2018/2/21

N2 - We herein report an efficient hybridization chain reaction (HCR)- and DNAzyme-based enzyme-free signal amplification for the detection of specific exosomal miRNAs in the culture medium of cancer cells and serum samples from cancer patients via the target-triggered self-assembly of the polymer DNAzyme nanostructure.

AB - We herein report an efficient hybridization chain reaction (HCR)- and DNAzyme-based enzyme-free signal amplification for the detection of specific exosomal miRNAs in the culture medium of cancer cells and serum samples from cancer patients via the target-triggered self-assembly of the polymer DNAzyme nanostructure.

UR - https://www.scopus.com/pages/publications/85041963660

U2 - 10.1039/c7an01691c

DO - 10.1039/c7an01691c

M3 - Journal article

C2 - 29362731

AN - SCOPUS:85041963660

SN - 0003-2654

VL - 143

SP - 813

EP - 816

JO - Analyst

JF - Analyst

IS - 4

ER -

Enzyme-free quantification of exosomal microRNA by the target-triggered assembly of the polymer DNAzyme nanostructure

Abstract

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