TY - JOUR
T1 - Enhanced microbial biodiesel production from lignocellulosic hydrolysates using yeast isolates
AU - Ananthi, V.
AU - Siva Prakash, G.
AU - Chang, Soon Woong
AU - Ravindran, Balasubramani
AU - Nguyen, Dinh Duc
AU - Vo, Dai Viet N.
AU - La, Duong Duc
AU - Bach, Quang Vu
AU - Wong, Jonathan W C
AU - Kumar Gupta, Sanjay
AU - Selvaraj, Arokiyaraj
AU - Arun, A.
N1 - Funding Information:
The authors acknowledge the financial support in general and instruments facilities sponsored by:, 1. Department of Science and Technology-Promotion of University Research and Scientific Excellence (DST-PURSE) (DST letter No.SR/PURSE phase 2/38(G), dt.21.02.2017), India. 2. RUSA ? Phase 2.0 grant sanctioned vide Letter No. F.24-51/2014-U, Policy (TNMulti-Gen), Dept. of Edn. Govt. of India, Dt.09.10.2018.
PY - 2019/11/15
Y1 - 2019/11/15
N2 - An effort in contributing to the reduction of fossil resource dependence along with utilization of lignocellulosic wastes, will be a beneficial approach in biofuel production. These approaches will aid in sustainable waste management and will contribute to economic feasibility. This study probed the exploitation of abundant, non-edible lignocellulosic wastes such as sugarcane bagasse and rice husk as a low-cost carbon source for the cultivation of oleaginous yeast isolates and lipid accumulation, respectively. The hydrolysis of the lignocellulose was explored by steam explosion alone to reduce the inflation of toxic compounds like hydroxy methyl furfural (HMF), furfural, and acetic acid. This optimization study investigated the effects of carbon sources (sugarcane bagasse hydrolysate, rice husk hydrolysate, and commercial glucose), nitrogen sources, and pH on biodiesel production using central composite design (CCD), and achieved a full factorial design. All the three variables were found to have a positive influence on biodiesel production. Greater biomass production was achieved by SY2 (Pichia kudriavzevii) with a value of −8.37 ± 0.067 g/L when sugarcane bagasse hydrolysate was used. Maximum lipid content of 37.99 ± 0.003% was attained by the strain G5 (Meyerozyma guilliermondii) upon using sugarcane bagasse hydrolysate in the production medium and higher lipid accumulation of 2.39 ± 0.003 g/L was attained by SY2 (Pichia kudriavzevii) upon using rice husk hydrolysate. Analysis of the kinematic properties of FAME (Fatty Acid Methyl Esters) was obtained using Biodiesel analyzer V1.1. software, and affirmed the values similar to the biodiesel standard values (EN 14214, ASTM, IS 15607). The kinematic studies used to figure out the quality of the biodiesel, revealed that FAME produced by SY2 (Pichia kudriavzevii) strain showed greater oxidation stability (37.17 h) and better kinematic viscosity (1.3084). These properties ensured that SY2 (Pichia kudriavzevii) strain was appropriate in producing exceptional quality biodiesel. Hence, the present investigation tried to prove the competency of the yeast isolates in sustainable biodiesel production by engaging agronomic wastes.
AB - An effort in contributing to the reduction of fossil resource dependence along with utilization of lignocellulosic wastes, will be a beneficial approach in biofuel production. These approaches will aid in sustainable waste management and will contribute to economic feasibility. This study probed the exploitation of abundant, non-edible lignocellulosic wastes such as sugarcane bagasse and rice husk as a low-cost carbon source for the cultivation of oleaginous yeast isolates and lipid accumulation, respectively. The hydrolysis of the lignocellulose was explored by steam explosion alone to reduce the inflation of toxic compounds like hydroxy methyl furfural (HMF), furfural, and acetic acid. This optimization study investigated the effects of carbon sources (sugarcane bagasse hydrolysate, rice husk hydrolysate, and commercial glucose), nitrogen sources, and pH on biodiesel production using central composite design (CCD), and achieved a full factorial design. All the three variables were found to have a positive influence on biodiesel production. Greater biomass production was achieved by SY2 (Pichia kudriavzevii) with a value of −8.37 ± 0.067 g/L when sugarcane bagasse hydrolysate was used. Maximum lipid content of 37.99 ± 0.003% was attained by the strain G5 (Meyerozyma guilliermondii) upon using sugarcane bagasse hydrolysate in the production medium and higher lipid accumulation of 2.39 ± 0.003 g/L was attained by SY2 (Pichia kudriavzevii) upon using rice husk hydrolysate. Analysis of the kinematic properties of FAME (Fatty Acid Methyl Esters) was obtained using Biodiesel analyzer V1.1. software, and affirmed the values similar to the biodiesel standard values (EN 14214, ASTM, IS 15607). The kinematic studies used to figure out the quality of the biodiesel, revealed that FAME produced by SY2 (Pichia kudriavzevii) strain showed greater oxidation stability (37.17 h) and better kinematic viscosity (1.3084). These properties ensured that SY2 (Pichia kudriavzevii) strain was appropriate in producing exceptional quality biodiesel. Hence, the present investigation tried to prove the competency of the yeast isolates in sustainable biodiesel production by engaging agronomic wastes.
KW - Lignocellulosic wastes
KW - Oleaginous
KW - Optimization biodiesel
KW - Rice husk
KW - Sugarcane bagasse
UR - http://www.scopus.com/inward/record.url?scp=85070589528&partnerID=8YFLogxK
U2 - 10.1016/j.fuel.2019.115932
DO - 10.1016/j.fuel.2019.115932
M3 - Journal article
AN - SCOPUS:85070589528
SN - 0016-2361
VL - 256
JO - Fuel
JF - Fuel
M1 - 115932
ER -
