Engineering of a genetically encodable fluorescent voltage sensor exploiting fast Ci-VSP voltage-sensing movements

Alicia Lundby; Hiroki Mutoh; Dimitar Dimitrov; Walther Akemann; Thomas Knöpfel

doi:10.1371/journal.pone.0002514

Engineering of a genetically encodable fluorescent voltage sensor exploiting fast Ci-VSP voltage-sensing movements

Alicia Lundby^*
, Hiroki Mutoh
, Dimitar Dimitrov
, Walther Akemann
, Thomas Knöpfel^*

^*Corresponding author for this work

Department of Physics

Research output: Contribution to journal › Journal article › peer-review

137 Citations (Scopus)

Abstract

Ci-VSP contains a voltage-sensing domain (VSD) homologous to that of voltage-gated potassium channels. Using charge displacement ('gating' current) measurements we show that voltage-sensing movements of this VSD can occur within 1 ms in mammalian membranes. Our analysis lead to development of a genetically encodable fluorescent protein voltage sensor (VSFP) in which the fast, voltage-dependent conformational changes of the Ci-VSP voltage sensor are transduced to similarly fast fluorescence read-outs.

Original language	English
Article number	e2514
Number of pages	5
Journal	PLoS ONE
Volume	3
Issue number	6
DOIs	https://doi.org/10.1371/journal.pone.0002514
Publication status	Published - 25 Jun 2008

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title = "Engineering of a genetically encodable fluorescent voltage sensor exploiting fast Ci-VSP voltage-sensing movements",

abstract = "Ci-VSP contains a voltage-sensing domain (VSD) homologous to that of voltage-gated potassium channels. Using charge displacement ('gating' current) measurements we show that voltage-sensing movements of this VSD can occur within 1 ms in mammalian membranes. Our analysis lead to development of a genetically encodable fluorescent protein voltage sensor (VSFP) in which the fast, voltage-dependent conformational changes of the Ci-VSP voltage sensor are transduced to similarly fast fluorescence read-outs.",

author = "Alicia Lundby and Hiroki Mutoh and Dimitar Dimitrov and Walther Akemann and Thomas Kn{\"o}pfel",

note = "Supported by RIKEN BSI intramural funds, NIH grant NS057631 and a stipend from Danish Cardiovascular Research Academy to AL. Publisher Copyright: {\textcopyright} 2008 Lundby et al.",

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AU - Lundby, Alicia

AU - Mutoh, Hiroki

AU - Dimitrov, Dimitar

AU - Akemann, Walther

AU - Knöpfel, Thomas

PY - 2008/6/25

Y1 - 2008/6/25

N2 - Ci-VSP contains a voltage-sensing domain (VSD) homologous to that of voltage-gated potassium channels. Using charge displacement ('gating' current) measurements we show that voltage-sensing movements of this VSD can occur within 1 ms in mammalian membranes. Our analysis lead to development of a genetically encodable fluorescent protein voltage sensor (VSFP) in which the fast, voltage-dependent conformational changes of the Ci-VSP voltage sensor are transduced to similarly fast fluorescence read-outs.

AB - Ci-VSP contains a voltage-sensing domain (VSD) homologous to that of voltage-gated potassium channels. Using charge displacement ('gating' current) measurements we show that voltage-sensing movements of this VSD can occur within 1 ms in mammalian membranes. Our analysis lead to development of a genetically encodable fluorescent protein voltage sensor (VSFP) in which the fast, voltage-dependent conformational changes of the Ci-VSP voltage sensor are transduced to similarly fast fluorescence read-outs.

UR - https://www.scopus.com/pages/publications/49649106720

U2 - 10.1371/journal.pone.0002514

DO - 10.1371/journal.pone.0002514

M3 - Journal article

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JO - PLoS ONE

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Engineering of a genetically encodable fluorescent voltage sensor exploiting fast Ci-VSP voltage-sensing movements

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