Endogenous production of protoporphyrin IX induced by 5-aminolevulinic acid in leukemia cells

Ji-Yao Chen*, Nai-Qi Mak, Nai-Hao Cheung, Rong-Neng Leung, Qian Peng

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

16 Citations (Scopus)


AIM: To explore the photosensitization of 5-aminolevulinic acid (ALA) in myeloid leukemia cell line. 

METHODS: Using the technique of fluorescence spectra, the ALA induced protoporphyrin IX (PpIX) was measured in myeloid leukemia JCS cells. Cofocal laser scanning microscopy (CLSM) combined with fluorescence organelle probe was used to detecte the localization of PpIX in JCS cells at the subcellular levels. MTT assay was used to measure the cell survival after light irradiation. 

RESULTS: ALA successfully produced endogenous PpIX in leukemia JCS cells. PpIX was observed to be distributed in the cytoplasm and mitochondria was exhibited as the one of binding sites of PpIX. As a photosensitizer, PpIX initiated photodynamic reaction after light irradiation and effectivily photodamaged leukemia cells. 

CONCLUSION: ALA-based photosensitization could be used for inactivation of leukemia cells.



结果:ALA成功的在白血病细胞中内源生成光敏药物原卟啉IX ;原卟啉IX分布在细胞核以外的细胞质中,线粒体是其的结合部位之一;经光照后此内源生成的原卟啉IX启动光敏化反应有效的灭活白血病细胞.


Original languageEnglish
Pages (from-to)163-168
Number of pages6
JournalActa Pharmacologica Sinica
Issue number2
Publication statusPublished - Feb 2001

Scopus Subject Areas

  • Pharmacology
  • Pharmacology (medical)

User-Defined Keywords

  • 5-aminolevulinic acid
  • Photosensitization
  • Protoporphyrin
  • 氨乙酸丙酸
  • 原卟啉
  • 光敏反应


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