AIM: To explore the photosensitization of 5-aminolevulinic acid (ALA) in myeloid leukemia cell line. METHODS: Using the technique of fluorescence spectra, the ALA induced protoporphyrin IX (PpIX) was measured in myeloid leukemia JCS cells. Cofocal laser scanning microscopy (CLSM) combined with fluorescence organelle probe was used to detecte the localization of PpIX in JCS cells at the subcellular levels. MTT assay was used to measure the cell survival after light irradiation. RESULTS: ALA successfully produced endogenous PpIX in leukemia JCS cells. PpIX was observed to be distributed in the cytoplasm and mitochondria was exhibited as the one of binding sites of PpIX. As a photosensitizer, PpIX initiated photodynamic reaction after light irradiation and effectivily photodamaged leukemia cells. CONCLUSION: ALA-based photosensitization could be used for inactivation of leukemia cells.
|Number of pages||6|
|Journal||Acta Pharmacologica Sinica|
|Publication status||Published - Feb 2001|
Scopus Subject Areas
- Pharmacology (medical)
- 5-aminolevulinic acid