Abstract
AIM: To explore the photosensitization of 5-aminolevulinic acid (ALA) in myeloid leukemia cell line.
METHODS: Using the technique of fluorescence spectra, the ALA induced protoporphyrin IX (PpIX) was measured in myeloid leukemia JCS cells. Cofocal laser scanning microscopy (CLSM) combined with fluorescence organelle probe was used to detecte the localization of PpIX in JCS cells at the subcellular levels. MTT assay was used to measure the cell survival after light irradiation.
RESULTS: ALA successfully produced endogenous PpIX in leukemia JCS cells. PpIX was observed to be distributed in the cytoplasm and mitochondria was exhibited as the one of binding sites of PpIX. As a photosensitizer, PpIX initiated photodynamic reaction after light irradiation and effectivily photodamaged leukemia cells.
CONCLUSION: ALA-based photosensitization could be used for inactivation of leukemia cells.
目的：探索用氨乙酸丙酸（ALA）对髄性白血病细胞进行光敏化灭活.
方法：用荧光光谱技术测量ALA在白血病细胞中内源生成光敏药物原卟啉IX;用共焦激光扫描荧光显微镜结合亚细胞器荧光探针探测其在JCS摘性白血病细胞中的分布;用MTT方法检测光敏化后的细胞存活.
结果：ALA成功的在白血病细胞中内源生成光敏药物原卟啉IX ;原卟啉IX分布在细胞核以外的细胞质中，线粒体是其的结合部位之一;经光照后此内源生成的原卟啉IX启动光敏化反应有效的灭活白血病细胞.
结论：基于ALA的光动力反应有潜力用于对白血病细胞的光敏化灭活.
| Original language | English |
|---|---|
| Pages (from-to) | 163-168 |
| Number of pages | 6 |
| Journal | Acta Pharmacologica Sinica |
| Volume | 22 |
| Issue number | 2 |
| Publication status | Published - Feb 2001 |
User-Defined Keywords
- 5-aminolevulinic acid
- Photosensitization
- Protoporphyrin
- 氨乙酸丙酸
- 原卟啉
- 光敏反应
