Enantioselective quenching of room-temperature phosphorescence lifetimes of proteins: Bovine and human serum albumins

Yanli Wei, Chuan Dong*, Diansheng Liu, Shaomin Shuang, Carmen W K HUIE

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

Enantioselective quenching of the room-temperature phosphorescence RTP lifetime of proteins was demonstrated due to the effects of various external chiral quenching agents. In the absence of quenchers, the RTP lifetimes for bovine serum albumin BSA and human serum albumin HSA were found to be 5.0 ± 0.2 and 4.0 ± 0.1 ms, respectively. The addition of various chiral quenchers three pairs of binaphthols and two pairs of β-blockers into the deoxygenated sample solutions containing BSA and HSA reduced their RTP lifetimes significantly, i.e., from ca. 4-5 ms in the absence to an average lifetime of ca. 1-2 ms in the presence of the chiral quenchers. For the R and S enantiomers examined, marked differences in RTP lifetimes were observed, i.e., ranging from ca. 20-29% for the binaphthols to ca. 14-16% for the β-blockers. Such findings could lead to a better understanding of the relationship between chirality, dynamics/conformational changes, and biological functions of proteins.

Original languageEnglish
Pages (from-to)761-764
Number of pages4
JournalBiomacromolecules
Volume8
Issue number3
DOIs
Publication statusPublished - Mar 2007

Scopus Subject Areas

  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

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