Enantiomeric spatiotemporal dynamics of nonpolar amino acids during mung bean germination by UPLC-MS and MALDI-TIMS-MS

Yanhui Zhong; Peisi Xie; Lei Guo; Zian Lin; Zongwei Cai

doi:10.1016/j.talanta.2025.128882

Enantiomeric spatiotemporal dynamics of nonpolar amino acids during mung bean germination by UPLC-MS and MALDI-TIMS-MS

Yanhui Zhong, Peisi Xie, Lei Guo, Zian Lin^*, Zongwei Cai^*

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

Abstract

Mung bean sprouting significantly modifies the composition of free amino acids (AAs), making studying its dynamic changes crucial for food nutrition, food processing, and plant metabolism. Utilizing derivatization with chiral α-methoxy-α-trifluoromethylphenylacetyl chloride, ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS) and matrix-assisted laser desorption/ionization-trapped ion mobility spectrometry-mass spectrometry (MALDI-TIMS-MS) methods were developed for enantiomeric AAs analysis. Mechanism study revealed that the structural differences of the derivatized AA enantiomers drive their separation. UPLC-MS with a C18 column resolved twelve nonpolar AAs within 30 min, achieving detection limits of 1.4–4.1 ng/L. MALDI-TIMS-MS allowed rapid separation and spatial mapping of the same set of AAs under fixed conditions with little sample preparation. The derivatization strategy provides a novel approach for chiral analysis and chiral metabolomics.

Original language	English
Article number	128882
Number of pages	9
Journal	Talanta
Volume	298, Part A
Early online date	19 Sept 2025
DOIs	https://doi.org/10.1016/j.talanta.2025.128882
Publication status	E-pub ahead of print - 19 Sept 2025

User-Defined Keywords

Chiral amino acids
MALDI-TIMS-MS
Mung bean
UPLC-MS
α-methoxy-α-trifluoromethylphenylacetyl chloride

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10.1016/j.talanta.2025.128882

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@article{b4bc135f5f5044b5ad61f6e535018c5a,

title = "Enantiomeric spatiotemporal dynamics of nonpolar amino acids during mung bean germination by UPLC-MS and MALDI-TIMS-MS",

abstract = "Mung bean sprouting significantly modifies the composition of free amino acids (AAs), making studying its dynamic changes crucial for food nutrition, food processing, and plant metabolism. Utilizing derivatization with chiral α-methoxy-α-trifluoromethylphenylacetyl chloride, ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS) and matrix-assisted laser desorption/ionization-trapped ion mobility spectrometry-mass spectrometry (MALDI-TIMS-MS) methods were developed for enantiomeric AAs analysis. Mechanism study revealed that the structural differences of the derivatized AA enantiomers drive their separation. UPLC-MS with a C18 column resolved twelve nonpolar AAs within 30 min, achieving detection limits of 1.4–4.1 ng/L. MALDI-TIMS-MS allowed rapid separation and spatial mapping of the same set of AAs under fixed conditions with little sample preparation. The derivatization strategy provides a novel approach for chiral analysis and chiral metabolomics.",

keywords = "Chiral amino acids, MALDI-TIMS-MS, Mung bean, UPLC-MS, α-methoxy-α-trifluoromethylphenylacetyl chloride",

author = "Yanhui Zhong and Peisi Xie and Lei Guo and Zian Lin and Zongwei Cai",

note = "This research was supported by the National Natural Science Foundation of China (22404023, and 22274021), Fuzhou University Testing Fund of precious apparatus (2025T020). Copyright {\textcopyright} 2025 Elsevier B.V. All rights reserved.",

year = "2025",

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doi = "10.1016/j.talanta.2025.128882",

language = "English",

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T1 - Enantiomeric spatiotemporal dynamics of nonpolar amino acids during mung bean germination by UPLC-MS and MALDI-TIMS-MS

AU - Zhong, Yanhui

AU - Xie, Peisi

AU - Guo, Lei

AU - Lin, Zian

AU - Cai, Zongwei

N1 - This research was supported by the National Natural Science Foundation of China (22404023, and 22274021), Fuzhou University Testing Fund of precious apparatus (2025T020). Copyright © 2025 Elsevier B.V. All rights reserved.

PY - 2025/9/19

Y1 - 2025/9/19

N2 - Mung bean sprouting significantly modifies the composition of free amino acids (AAs), making studying its dynamic changes crucial for food nutrition, food processing, and plant metabolism. Utilizing derivatization with chiral α-methoxy-α-trifluoromethylphenylacetyl chloride, ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS) and matrix-assisted laser desorption/ionization-trapped ion mobility spectrometry-mass spectrometry (MALDI-TIMS-MS) methods were developed for enantiomeric AAs analysis. Mechanism study revealed that the structural differences of the derivatized AA enantiomers drive their separation. UPLC-MS with a C18 column resolved twelve nonpolar AAs within 30 min, achieving detection limits of 1.4–4.1 ng/L. MALDI-TIMS-MS allowed rapid separation and spatial mapping of the same set of AAs under fixed conditions with little sample preparation. The derivatization strategy provides a novel approach for chiral analysis and chiral metabolomics.

AB - Mung bean sprouting significantly modifies the composition of free amino acids (AAs), making studying its dynamic changes crucial for food nutrition, food processing, and plant metabolism. Utilizing derivatization with chiral α-methoxy-α-trifluoromethylphenylacetyl chloride, ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS) and matrix-assisted laser desorption/ionization-trapped ion mobility spectrometry-mass spectrometry (MALDI-TIMS-MS) methods were developed for enantiomeric AAs analysis. Mechanism study revealed that the structural differences of the derivatized AA enantiomers drive their separation. UPLC-MS with a C18 column resolved twelve nonpolar AAs within 30 min, achieving detection limits of 1.4–4.1 ng/L. MALDI-TIMS-MS allowed rapid separation and spatial mapping of the same set of AAs under fixed conditions with little sample preparation. The derivatization strategy provides a novel approach for chiral analysis and chiral metabolomics.

KW - Chiral amino acids

KW - MALDI-TIMS-MS

KW - Mung bean

KW - UPLC-MS

KW - α-methoxy-α-trifluoromethylphenylacetyl chloride

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DO - 10.1016/j.talanta.2025.128882

M3 - Journal article

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AN - SCOPUS:105016306570

SN - 0039-9140

VL - 298, Part A

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JF - Talanta

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ER -

Enantiomeric spatiotemporal dynamics of nonpolar amino acids during mung bean germination by UPLC-MS and MALDI-TIMS-MS

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